Photoacoustic signal characterization of cancer treatment response: Correlation with changes in tumor oxygenation
Photoacoustic signal characterization of cancer treatment response: Correlation with changes in tumor oxygenation
Frequency analysis of the photoacoustic radiofrequency signals and oxygen saturation estimates were used to monitor the in-vivo response of a novel, thermosensitive liposome treatment. The liposome encapsulated doxorubicin (HaT-DOX) releasing it rapidly (<20 s) when the tumor was exposed to mild hyperthermia (43 °C). Photoacoustic imaging (VevoLAZR, 750/850 nm, 40 MHz) of EMT-6 breast cancer tumors was performed 30 min pre- and post-treatment and up to 7 days post-treatment (at 2/5/24 h timepoints). HaT-DOX-treatment responders exhibited on average a 22% drop in oxygen saturation 2 h post-treatment and a decrease (45% at 750 nm and 73% at 850 nm) in the slope of the normalized PA frequency spectra. The spectral slope parameter correlated with treatment-induced hemorrhaging which increased the optical absorber effective size via interstitial red blood cell leakage. Combining frequency analysis and oxygen saturation estimates differentiated treatment responders from non-responders/control animals by probing the treatment-induced structural changes of blood vessel.
Cancer treatment monitoring, Oxygen saturation, Photoacoustic and ultrasound tissue characterization, Radiofrequency analysis, Tumor blood vessels
25-35
Hysi, Eno
930da6bf-baf4-4334-8cc8-ee0264476b32
Wirtzfeld, Lauren A.
e46c0f07-84dc-4439-b349-36f81c49c413
May, Jonathan P.
b54a262b-9f32-4435-8866-3b9c495294f3
Undzys, Elijus
226e7879-8bef-4715-9e72-d405e59d6dcb
Li, Shyh Dar
9a960896-36e7-4eb8-bd39-55580c60e36d
Kolios, Michael C.
524bd088-1670-4d3c-9817-5298a4b1eebd
1 March 2017
Hysi, Eno
930da6bf-baf4-4334-8cc8-ee0264476b32
Wirtzfeld, Lauren A.
e46c0f07-84dc-4439-b349-36f81c49c413
May, Jonathan P.
b54a262b-9f32-4435-8866-3b9c495294f3
Undzys, Elijus
226e7879-8bef-4715-9e72-d405e59d6dcb
Li, Shyh Dar
9a960896-36e7-4eb8-bd39-55580c60e36d
Kolios, Michael C.
524bd088-1670-4d3c-9817-5298a4b1eebd
Hysi, Eno, Wirtzfeld, Lauren A., May, Jonathan P., Undzys, Elijus, Li, Shyh Dar and Kolios, Michael C.
(2017)
Photoacoustic signal characterization of cancer treatment response: Correlation with changes in tumor oxygenation.
Photoacoustics, 5, .
(doi:10.1016/j.pacs.2017.03.003).
Abstract
Frequency analysis of the photoacoustic radiofrequency signals and oxygen saturation estimates were used to monitor the in-vivo response of a novel, thermosensitive liposome treatment. The liposome encapsulated doxorubicin (HaT-DOX) releasing it rapidly (<20 s) when the tumor was exposed to mild hyperthermia (43 °C). Photoacoustic imaging (VevoLAZR, 750/850 nm, 40 MHz) of EMT-6 breast cancer tumors was performed 30 min pre- and post-treatment and up to 7 days post-treatment (at 2/5/24 h timepoints). HaT-DOX-treatment responders exhibited on average a 22% drop in oxygen saturation 2 h post-treatment and a decrease (45% at 750 nm and 73% at 850 nm) in the slope of the normalized PA frequency spectra. The spectral slope parameter correlated with treatment-induced hemorrhaging which increased the optical absorber effective size via interstitial red blood cell leakage. Combining frequency analysis and oxygen saturation estimates differentiated treatment responders from non-responders/control animals by probing the treatment-induced structural changes of blood vessel.
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Published date: 1 March 2017
Keywords:
Cancer treatment monitoring, Oxygen saturation, Photoacoustic and ultrasound tissue characterization, Radiofrequency analysis, Tumor blood vessels
Identifiers
Local EPrints ID: 455593
URI: http://eprints.soton.ac.uk/id/eprint/455593
ISSN: 2213-5979
PURE UUID: 5fa71236-9b6d-4f63-bd66-da1949f08d51
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Date deposited: 28 Mar 2022 16:44
Last modified: 18 Mar 2024 03:49
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Contributors
Author:
Eno Hysi
Author:
Lauren A. Wirtzfeld
Author:
Elijus Undzys
Author:
Shyh Dar Li
Author:
Michael C. Kolios
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