Postembryonic lineages of the Drosophila ventral nervous system: Neuroglian expression reveals the adult hemilineage associated fiber tracts in the adult thoracic neuromeres
Postembryonic lineages of the Drosophila ventral nervous system: Neuroglian expression reveals the adult hemilineage associated fiber tracts in the adult thoracic neuromeres
During larval life most of the thoracic neuroblasts (NBs) in Drosophila undergo a second phase of neurogenesis to generate adult-specific neurons that remain in an immature, developmentally stalled state until pupation. Using a combination of MARCM and immunostaining with a neurotactin antibody, Truman et al. (2004; Development 131:5167-5184) identified 24 adult-specific NB lineages within each thoracic hemineuromere of the larval ventral nervous system (VNS), but because of the neurotactin labeling of lineage tracts disappearing early in metamorphosis, they were unable extend the identification of these lineages into the adult. Here we show that immunostaining with an antibody against the cell adhesion molecule neuroglian reveals the same larval secondary lineage projections through metamorphosis and bfy identifying each neuroglian-positive tract at selected stages we have traced the larval hemilineage tracts for all three thoracic neuromeres through metamorphosis into the adult. To validate tract identifications we used the genetic toolkit developed by Harris et al. (2015; Elife 4) to preserve hemilineage-specific GAL4 expression patterns from larval into the adult stage. The immortalized expression proved a powerful confirmation of the analysis of the neuroglian scaffold. This work has enabled us to directly link the secondary, larval NB lineages to their adult counterparts. The data provide an anatomical framework that 1) makes it possible to assign most neurons to their parent lineage and 2) allows more precise definitions of the neuronal organization of the adult VNS based in developmental units/rules. J. Comp. Neurol. 524:2677-2695, 2016. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.
Age Factors, Animals, Animals, Genetically Modified, Cell Adhesion Molecules, Neuronal/biosynthesis, Cell Lineage/physiology, Drosophila, Drosophila Proteins/biosynthesis, Genetic Variation/physiology, Nerve Fibers/metabolism, Nervous System/embryology, Neurogenesis/physiology
2677-95
Shepherd, David
11aa6858-d19c-4450-82ff-11dff9dcd9c4
Harris, Robin
b9100282-7090-4d51-9802-2193d697b68a
Williams, Darren W.
604e70da-4237-4069-87b0-6dff0917ca49
Truman, James W.
558a187a-bf3a-4ffd-a6a4-c1f8449f5938
1 September 2016
Shepherd, David
11aa6858-d19c-4450-82ff-11dff9dcd9c4
Harris, Robin
b9100282-7090-4d51-9802-2193d697b68a
Williams, Darren W.
604e70da-4237-4069-87b0-6dff0917ca49
Truman, James W.
558a187a-bf3a-4ffd-a6a4-c1f8449f5938
Shepherd, David, Harris, Robin, Williams, Darren W. and Truman, James W.
(2016)
Postembryonic lineages of the Drosophila ventral nervous system: Neuroglian expression reveals the adult hemilineage associated fiber tracts in the adult thoracic neuromeres.
Journal of Comparative Neurology, 524 (13), .
(doi:10.1002/cne.23988).
Abstract
During larval life most of the thoracic neuroblasts (NBs) in Drosophila undergo a second phase of neurogenesis to generate adult-specific neurons that remain in an immature, developmentally stalled state until pupation. Using a combination of MARCM and immunostaining with a neurotactin antibody, Truman et al. (2004; Development 131:5167-5184) identified 24 adult-specific NB lineages within each thoracic hemineuromere of the larval ventral nervous system (VNS), but because of the neurotactin labeling of lineage tracts disappearing early in metamorphosis, they were unable extend the identification of these lineages into the adult. Here we show that immunostaining with an antibody against the cell adhesion molecule neuroglian reveals the same larval secondary lineage projections through metamorphosis and bfy identifying each neuroglian-positive tract at selected stages we have traced the larval hemilineage tracts for all three thoracic neuromeres through metamorphosis into the adult. To validate tract identifications we used the genetic toolkit developed by Harris et al. (2015; Elife 4) to preserve hemilineage-specific GAL4 expression patterns from larval into the adult stage. The immortalized expression proved a powerful confirmation of the analysis of the neuroglian scaffold. This work has enabled us to directly link the secondary, larval NB lineages to their adult counterparts. The data provide an anatomical framework that 1) makes it possible to assign most neurons to their parent lineage and 2) allows more precise definitions of the neuronal organization of the adult VNS based in developmental units/rules. J. Comp. Neurol. 524:2677-2695, 2016. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.
Text
J of Comparative Neurology - 2016 - Shepherd - Postembryonic lineages of the Drosophila ventral nervous system Neuroglian
- Version of Record
More information
Accepted/In Press date: 12 February 2016
e-pub ahead of print date: 9 March 2016
Published date: 1 September 2016
Keywords:
Age Factors, Animals, Animals, Genetically Modified, Cell Adhesion Molecules, Neuronal/biosynthesis, Cell Lineage/physiology, Drosophila, Drosophila Proteins/biosynthesis, Genetic Variation/physiology, Nerve Fibers/metabolism, Nervous System/embryology, Neurogenesis/physiology
Identifiers
Local EPrints ID: 455599
URI: http://eprints.soton.ac.uk/id/eprint/455599
ISSN: 0021-9967
PURE UUID: d12e41cd-cc8e-4bcf-ac88-20950c66bb87
Catalogue record
Date deposited: 28 Mar 2022 16:47
Last modified: 06 Aug 2024 01:52
Export record
Altmetrics
Contributors
Author:
Robin Harris
Author:
Darren W. Williams
Author:
James W. Truman
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
