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Production and characterization of monoclonal antibodies to bovid herpesvirus-4

Production and characterization of monoclonal antibodies to bovid herpesvirus-4
Production and characterization of monoclonal antibodies to bovid herpesvirus-4
Thirty-five hybridoma cell lines secreting monoclonal antibodies (Mabs) against bovid herpes-virus-4 (BHV-4) strain V. Test were produced. These hybrid cells resulted from the fusion of SP2/0 myeloma cells with splenocytes of BALB/c mice previously immunized with purified BHV-4.

A modified indirect fluorescent antibody test (IFAT) was applied as a screening procedure and was compared with an indirect enzyme-linked immunosorbent assay. The selected Mabs were tested by the same IFAT against a panel of BHV-4 field isolates and against bovine herpesvirus-1, bovine herpesvirus-2 and alcelaphine herpesvirus-1 (AHV-1). Comparison of BHV-4 field isolates with Mabs confirmed their close antigenic relationships, but slight antigenic differences were observed between different isolates. One of the Mabs also reacted against AHV-1, indicating an antigenic relationship between BHV-4 and AHV-1. None of the Mabs reacting with BHV-4 possessed neutralizing activity against the strain used for immunization.
0378-1135
305-315
Boulanger, Denise
c226ad99-9c9a-485a-b480-42fb8799120f
Boulanger, Denise
c226ad99-9c9a-485a-b480-42fb8799120f

Boulanger, Denise (1989) Production and characterization of monoclonal antibodies to bovid herpesvirus-4. Veterinary Microbiology, 19 (4), 305-315. (doi:10.1016/0378-1135(89)90096-5).

Record type: Article

Abstract

Thirty-five hybridoma cell lines secreting monoclonal antibodies (Mabs) against bovid herpes-virus-4 (BHV-4) strain V. Test were produced. These hybrid cells resulted from the fusion of SP2/0 myeloma cells with splenocytes of BALB/c mice previously immunized with purified BHV-4.

A modified indirect fluorescent antibody test (IFAT) was applied as a screening procedure and was compared with an indirect enzyme-linked immunosorbent assay. The selected Mabs were tested by the same IFAT against a panel of BHV-4 field isolates and against bovine herpesvirus-1, bovine herpesvirus-2 and alcelaphine herpesvirus-1 (AHV-1). Comparison of BHV-4 field isolates with Mabs confirmed their close antigenic relationships, but slight antigenic differences were observed between different isolates. One of the Mabs also reacted against AHV-1, indicating an antigenic relationship between BHV-4 and AHV-1. None of the Mabs reacting with BHV-4 possessed neutralizing activity against the strain used for immunization.

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Published date: 1 April 1989

Identifiers

Local EPrints ID: 455686
URI: http://eprints.soton.ac.uk/id/eprint/455686
ISSN: 0378-1135
PURE UUID: 5496a8ea-5c06-47f4-839b-a391e945ef30
ORCID for Denise Boulanger: ORCID iD orcid.org/0000-0003-1000-7313

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Date deposited: 30 Mar 2022 16:53
Last modified: 31 Mar 2022 01:38

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Author: Denise Boulanger ORCID iD

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