A monoclonal ELISA for bovine viral diarrhoea pestivirus antigen detection in persistently infected cattle
A monoclonal ELISA for bovine viral diarrhoea pestivirus antigen detection in persistently infected cattle
Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies raised against the 48-kDa glycoprotein and the -kDa protein was developed for detecting antigens in leucocytes of 3 persistently BVDV-infected calves. The test is simple, sensitive and rapid. Moreover the same ELISA was able to recognise Belgian field isolates of BVDV. These results show that the test can be applied in the field.
177-188
Mignon, B.
dc3aa7e5-48c1-4968-9539-af763b9f9301
Dubuisson, J.
f4ee6eb2-6a1e-4b0c-9227-de7987ea32c1
Baranowski, E.
906d5146-f8a7-471b-b0f2-c17a4448fe08
Koromyslov, I.
b901e2ca-c92f-4f76-9b5f-6a1e77b241d5
Ernst, E.
3978447d-2318-4c4f-81bd-84fa8e25dbdc
Boulanger, D.
c226ad99-9c9a-485a-b480-42fb8799120f
Waxweiler, S.
16f9fe28-a8df-4f83-9da4-c6f72ad26fc6
Pastoret, P.p.
ffb5a4df-e119-4396-9fc1-459327fa589d
1 November 1991
Mignon, B.
dc3aa7e5-48c1-4968-9539-af763b9f9301
Dubuisson, J.
f4ee6eb2-6a1e-4b0c-9227-de7987ea32c1
Baranowski, E.
906d5146-f8a7-471b-b0f2-c17a4448fe08
Koromyslov, I.
b901e2ca-c92f-4f76-9b5f-6a1e77b241d5
Ernst, E.
3978447d-2318-4c4f-81bd-84fa8e25dbdc
Boulanger, D.
c226ad99-9c9a-485a-b480-42fb8799120f
Waxweiler, S.
16f9fe28-a8df-4f83-9da4-c6f72ad26fc6
Pastoret, P.p.
ffb5a4df-e119-4396-9fc1-459327fa589d
Mignon, B., Dubuisson, J., Baranowski, E., Koromyslov, I., Ernst, E., Boulanger, D., Waxweiler, S. and Pastoret, P.p.
(1991)
A monoclonal ELISA for bovine viral diarrhoea pestivirus antigen detection in persistently infected cattle.
Journal of Virological Methods, 35 (2), .
(doi:10.1016/0166-0934(91)90133-K).
Abstract
Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies raised against the 48-kDa glycoprotein and the -kDa protein was developed for detecting antigens in leucocytes of 3 persistently BVDV-infected calves. The test is simple, sensitive and rapid. Moreover the same ELISA was able to recognise Belgian field isolates of BVDV. These results show that the test can be applied in the field.
This record has no associated files available for download.
More information
Published date: 1 November 1991
Identifiers
Local EPrints ID: 455690
URI: http://eprints.soton.ac.uk/id/eprint/455690
ISSN: 0166-0934
PURE UUID: 7c10aaf1-9bfc-411d-885e-73e84b3afb04
Catalogue record
Date deposited: 30 Mar 2022 16:55
Last modified: 17 Mar 2024 02:57
Export record
Altmetrics
Contributors
Author:
B. Mignon
Author:
J. Dubuisson
Author:
E. Baranowski
Author:
I. Koromyslov
Author:
E. Ernst
Author:
D. Boulanger
Author:
S. Waxweiler
Author:
P.p. Pastoret
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics