READ ME File For ‘Chapter 3, 4 and 5 Data Sets’

Dataset DOI:  https://doi.org/10.5258/SOTON/D2184

ReadMe Author: Nikita Karra, University of Southampton ORCID ID https://orcid.org/0000-0003-2250-7438

This dataset supports the thesis entitled “Development of an integrated aerosol drug delivery device for an in vitro lung on a chip”

AWARDED BY: University of Southampton
DATE OF AWARD: 2022

DESCRIPTION OF THE DATA
This data set includes excel files, graphed prism files and images related to experiments using the Airway Barrier on a Chip platform in Chapter 3, to the development and optimisation of the Surface Acoustic Wave based Drug Delivery Device in Chapter 4 and to the work generated from the integration of the drug delivery device with the airway barrier on a chip platform in Chapter 5.

Experiments:
- The growth of 16HBE14o- cells within the platform using different membranes.
- The growth and challenge of 16HBE14o- cels within the platform using dsRNA viral mimic poly I:C and fluticasone. (Experiments conducted in collaboration with Joao Fernandes).
- The preliminary experiments for SAW Chip Actuation (Time, Temperature, Voltage, Aerosol Generation)
- The data relating to nebulisation (rate, sample supply comparisons (droplet vs fluidic))
- The data relating to aerosol characterisation (distribution on a surface and size of aerosol droplets)
- The data associated with the static viral dsRNA analogue and corticosteroid stimulation using 16HBE14O- cells.
- The data associated with the static viral dsRNA analogue and extracellular vesicle stimulation using 16HBE14o- cells.
- The data associated with the static TNFa and dexamethasone stimulation of BCi cells.
- The data associated with the dynamic viral dsRNA analogue and corticosteroid stimulation using 16HBE14o- cells within the airway barrier on a chip platform.

The data was collected using a bespoke impedance analyser (Joel Bowring) alongside a script to covert the impedance data into a comparable ohms.cm2 format (Joao Fernandes), a voltohmmeter and a confocal microscope, transmission electron microscope, a droplet shape analyser, oscilloscope, thermocouple, malvern spraytech analyser. Data was statistically analysed using GraphPad prism. 

This dataset contains:
The corresponding data sets (excel, prism, lsm) and images for the following figures in Chapter 3: Figure 3.9 to 3.19. 

The corresponding data sets (excel, prism) and images and videos for the following figures in Chapter 4: Figure 4.4, 4.5, 4.7 to 4.12, 4.14 to 4.16, 4.18, 4.19, 4.22 to 4.24. In addition to the following related figures in the appendix: appendix 1 to appendix 13.

The corresponding data sets (excel, prism) and images for the following figures in Chapter 5: Figure 5.5 to 5.21. In addition to the following related figures in the appendix: appendix 43 to appendix 47, appendix 48 to appendix 50.

Date of data collection: Sept 2017 to October 2021

Related projects/Funders:
J.R.C.C Fernandes, Development of an organ on a chip microfluidic platform with real-time viability sensing capabilities. University of Southampton, Southampton, UK. 

The vesicle work was funded by EV Foundry (Grant Agreement Number: 801367)

Related publication:
Accepted
J. Fernandes, N.Karra, J. Bowring, R. Reale, J. James, C. Blume, T.J. Pell, W.C. Rowan, D.E. Davies, E.J. Swindle, H. Morgan, “Real-Time Monitoring of Epithelial Barrier Function By Impedance Spectroscopy in a Microfluidic Platform” Lab Chip, 2022

Under Review
N. Karra, M.J.C. Van Herwijnen, M.H.M. Wauben, E.J. Swindle, H. Morgan, “Human Milk Extracellular Vesicles Preserve Bronchial Epithelial Barrier Integrity and Reduce TLR-3 Induced Inflammation,” Journal of Extracellular Biology, 2022

In Preparation
N. Karra, J. Fernandes, E.J. Swindle, H. Morgan, “Integrating an Aerosolised Drug Delivery Device with Conventional Static Cultures and a Dynamic Airway Barrier Microphysiological System,” Lab Chip.

N. Karra, J. Fernandes, J. James, E.J. Swindle, H. Morgan, “Real-Time Analysis of Cell Growth on Different Membranes Using an ‘Airway Barrier on a Chip Platform’ Compared to Conventional Static Cultures,”Organs-on-a-Chip

Date that the file was created: April, 2022