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Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase

Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase
Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase
The Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I333 intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises stereoselective deprotonation and subsequent substrate assisted phosphate elimination, central to PLP biosynthesis.
2633-0679
227-230
Rodrigues, Matthew
8bb144f8-e917-457c-bb94-465bc225f961
Giri, Nitai
afcf6487-3951-4566-b8df-10d15ed64027
Royant, Antoine
96dd9b8f-12f9-4b8f-a80b-02f76a0a41de
Zhang, Yang
01fd380e-d1df-4465-b508-d714ee098f2d
Bolton, Rachel, Ann
ef622c55-8cf1-46e0-b3dd-97c967dce111
Evans, Gwyndaf
46cb660a-bce7-4c62-8afc-de2a197ed02c
Ealick, Steven E.
2cf05a29-f393-40a9-be87-06780885291e
Begley, Tadhg P.
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Tews, Ivo
9117fc5e-d01c-4f8d-a734-5b14d3eee8dd
Rodrigues, Matthew
8bb144f8-e917-457c-bb94-465bc225f961
Giri, Nitai
afcf6487-3951-4566-b8df-10d15ed64027
Royant, Antoine
96dd9b8f-12f9-4b8f-a80b-02f76a0a41de
Zhang, Yang
01fd380e-d1df-4465-b508-d714ee098f2d
Bolton, Rachel, Ann
ef622c55-8cf1-46e0-b3dd-97c967dce111
Evans, Gwyndaf
46cb660a-bce7-4c62-8afc-de2a197ed02c
Ealick, Steven E.
2cf05a29-f393-40a9-be87-06780885291e
Begley, Tadhg P.
7c3f0e44-7190-4ac3-a1df-0b23be54097b
Tews, Ivo
9117fc5e-d01c-4f8d-a734-5b14d3eee8dd

Rodrigues, Matthew, Giri, Nitai, Royant, Antoine, Zhang, Yang, Bolton, Rachel, Ann, Evans, Gwyndaf, Ealick, Steven E., Begley, Tadhg P. and Tews, Ivo (2022) Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase. RSC Chemical Biology, 3 (2), 227-230. (doi:10.1039/D1CB00160D).

Record type: Article

Abstract

The Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I333 intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises stereoselective deprotonation and subsequent substrate assisted phosphate elimination, central to PLP biosynthesis.

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Accepted/In Press date: 25 October 2021
e-pub ahead of print date: 25 October 2021
Published date: 9 February 2022
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Identifiers

Local EPrints ID: 456507
URI: http://eprints.soton.ac.uk/id/eprint/456507
DOI: doi:10.1039/D1CB00160D
ISSN: 2633-0679
PURE UUID: ffd911be-7e2b-45e5-96d7-bf041b31b6ff
ORCID for Ivo Tews: ORCID iD orcid.org/0000-0002-4704-1139

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Date deposited: 04 May 2022 16:48
Last modified: 12 Jul 2024 01:47

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Contributors

Author: Matthew Rodrigues
Author: Nitai Giri
Author: Antoine Royant
Author: Yang Zhang
Author: Rachel, Ann Bolton
Author: Gwyndaf Evans
Author: Steven E. Ealick
Author: Tadhg P. Begley
Author: Ivo Tews ORCID iD

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