РИБОСОМНАЯ РНК МЕЧНИКОВЕЛЛИД В ТРАНСКРИПТОМАХ ГРЕГАРИН И РДНК МИКРОСПОРИДИЙ SENSU LATO В МЕТАГЕНОМАХ

Mikhailov, K., Nassonova, E. S., Shishkin, Y. A., Paskerova, G. G., Simdyanov, T. G., Yudina, V. A., Smirnov, A., Janouskovec, J. and Aleoshin, V. V. (2021) РИБОСОМНАЯ РНК МЕЧНИКОВЕЛЛИД В ТРАНСКРИПТОМАХ ГРЕГАРИН И РДНК МИКРОСПОРИДИЙ SENSU LATO В МЕТАГЕНОМАХ. Zhurnal Obshchei Biologii. (doi:10.31857/S0044459621030040).

Abstract

n the metagenomes, transcriptomes, and amplicon libraries used for metastrichoding, numerous nucleotide sequences of sensu lato microsporidiums are present, mainly related to "Cryptomycota" (Rozellida, Rozellomycota, Rozellosporidia, treated herein as synonyms). In this report, we describe the rDNA sequences found in the transcriptomes of unicellular ones from the Apicomplexa group (Alveolata) belonging to hyperparasytic microsporidium-swordsmands. It was found that the transcript of eugregarin Polyrhabdina sp. (GenBank SRX6640468) contaminated with cDNA Metchnikovella incurvata, the transcript of the archigregarin Selenidium pygospionis (GenBank SRX6640459) contaminated with cDNA Metchnikovella dogieli, and in the transcriptome of the blastogregarina Siedleckia cf. nematoides (GenBank SRX6640464) found cDNA descending from a species from the undescribed family mechnicellids. Taking into account the covariance nucleotide substitutions, the secondary structure of the "ITS2" region of identified and unidentified swordsmenellids was modeled. Based on the predicted secondary structure of rRNA, mapping of readings from cDNA libraries, absence of endoribonuclease Las1 (PF04031), it is concluded that there is no processing of ITS2 and the presence of a covalent relationship of mature "5.8S" and "28S"-like (LSU) rRNA swordsmenellids in the same way as is known for other members of the group of typical microsporidiums. Several previously proposed (Chytridiopsis typographi, BAQA065) and new candidates for the role of a sister group of microsporidium sensu stricto were considered. The probable contribution of neutral and selective processes to the evolution of ultra-small microsporidium rRNA genes and ultra-long rRNA genes with group I introns of parasitic and lichen fungi is discussed.

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Contributors

Author: J. Janouskovec

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