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Anti-inflammatory and antimicrobial efficacy of electrospun nanofiber films for intracanal drug delivery

Anti-inflammatory and antimicrobial efficacy of electrospun nanofiber films for intracanal drug delivery
Anti-inflammatory and antimicrobial efficacy of electrospun nanofiber films for intracanal drug delivery
In an effort to combat the effects of endodontic infection, root canal treatment aims to remove endodontic bacteria. Nevertheless, endodontic treatment failure may occur due to microbial persistence and reinfection. Guidelines on dental infection management recommend triple antibiotic paste (TAP), a mixture of ciprofloxacin, metronidazole and minocycline, as an intra-canal medication for the eradication of endodontic bacteria1. Although the antimicrobial efficacy of the previous mentioned conventional remedy is generally recognized, they may produce some complications such as bacterial resistance, tissue irritation, stem cell toxicity and teeth discoloration due to its acidic nature. These limitations gave prominence to the use of nanoparticle-based irrigants, such as silver (Ag) or zinc oxide (ZnO) nanoparticles (NPs), both having a similar mode of bactericidal action. Equally important to eliminating endodontic microbial infection is the management of inflammation caused by bacterial infections. Ketoprofen is one of the most widely used nonsteroidal anti-inflammatory agents (NSAIDs) administered orally for the management of inflammation and postoperative endodontic pain4. To this extent, In the current study a rapidly dissolving electrospun film was developed for the intracanal co-administration of an antimicrobial (ZnO NPs) and an anti-inflammatory (ketoprofen) agent.

In the current study, polymeric fast-disintegrating films were successfully developed to address the antimicrobial and anti-inflammatory requirements for root canal treatment. The electrospun films showed good in vitro anti-inflammatory action on LPS-stimulated RAW 264.7 cells and adequate antimicrobial efficacy against E. faecalis, one of the most frequent species present in post-treatment disease. The antimicrobial efficacy of the nanofiber films was verified not only in vitro but also ex vivo in a human tooth model experimentally infected with E. faecalis. The combination of anti-inflammatory and antimicrobial activity to be exerted directly at the site of action shows a very appealing application for the treatment of periradicular infections and endodontic lesions.
Chachlioutaki, Konstantina
aa15e449-9ebc-4a23-a5a3-4a0a1327fc8e
Karavasili, Christina
f174d197-8ba7-4765-adc8-99fa2f1c86fa
Elisavet Adamoudi, Elisavet
1a092bc0-bb61-4839-80f5-261b9f079b74
Katsamenis, Orestis L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Bakopoulou, Athina
c0e871af-bc82-4232-ae1d-18429e80c737
Fatouros, Dimitrios G.
4a716c96-a8ba-4fbf-b3db-3b2a3a2794c4
Chachlioutaki, Konstantina
aa15e449-9ebc-4a23-a5a3-4a0a1327fc8e
Karavasili, Christina
f174d197-8ba7-4765-adc8-99fa2f1c86fa
Elisavet Adamoudi, Elisavet
1a092bc0-bb61-4839-80f5-261b9f079b74
Katsamenis, Orestis L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Bakopoulou, Athina
c0e871af-bc82-4232-ae1d-18429e80c737
Fatouros, Dimitrios G.
4a716c96-a8ba-4fbf-b3db-3b2a3a2794c4

Chachlioutaki, Konstantina, Karavasili, Christina, Elisavet Adamoudi, Elisavet, Katsamenis, Orestis L., Bakopoulou, Athina and Fatouros, Dimitrios G. (2022) Anti-inflammatory and antimicrobial efficacy of electrospun nanofiber films for intracanal drug delivery. 13th World Meeting<br/>on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, Ahoy conference centre, Rotterdam, Netherlands. 28 - 31 Mar 2022.

Record type: Conference or Workshop Item (Poster)

Abstract

In an effort to combat the effects of endodontic infection, root canal treatment aims to remove endodontic bacteria. Nevertheless, endodontic treatment failure may occur due to microbial persistence and reinfection. Guidelines on dental infection management recommend triple antibiotic paste (TAP), a mixture of ciprofloxacin, metronidazole and minocycline, as an intra-canal medication for the eradication of endodontic bacteria1. Although the antimicrobial efficacy of the previous mentioned conventional remedy is generally recognized, they may produce some complications such as bacterial resistance, tissue irritation, stem cell toxicity and teeth discoloration due to its acidic nature. These limitations gave prominence to the use of nanoparticle-based irrigants, such as silver (Ag) or zinc oxide (ZnO) nanoparticles (NPs), both having a similar mode of bactericidal action. Equally important to eliminating endodontic microbial infection is the management of inflammation caused by bacterial infections. Ketoprofen is one of the most widely used nonsteroidal anti-inflammatory agents (NSAIDs) administered orally for the management of inflammation and postoperative endodontic pain4. To this extent, In the current study a rapidly dissolving electrospun film was developed for the intracanal co-administration of an antimicrobial (ZnO NPs) and an anti-inflammatory (ketoprofen) agent.

In the current study, polymeric fast-disintegrating films were successfully developed to address the antimicrobial and anti-inflammatory requirements for root canal treatment. The electrospun films showed good in vitro anti-inflammatory action on LPS-stimulated RAW 264.7 cells and adequate antimicrobial efficacy against E. faecalis, one of the most frequent species present in post-treatment disease. The antimicrobial efficacy of the nanofiber films was verified not only in vitro but also ex vivo in a human tooth model experimentally infected with E. faecalis. The combination of anti-inflammatory and antimicrobial activity to be exerted directly at the site of action shows a very appealing application for the treatment of periradicular infections and endodontic lesions.

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More information

Published date: 28 March 2022
Venue - Dates: 13th World Meeting<br/>on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, Ahoy conference centre, Rotterdam, Netherlands, 2022-03-28 - 2022-03-31

Identifiers

Local EPrints ID: 456561
URI: http://eprints.soton.ac.uk/id/eprint/456561
PURE UUID: b7b68d0e-b1ef-4499-b205-e73233f4947f
ORCID for Orestis L. Katsamenis: ORCID iD orcid.org/0000-0003-4367-4147

Catalogue record

Date deposited: 05 May 2022 16:33
Last modified: 01 Sep 2022 01:43

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Contributors

Author: Konstantina Chachlioutaki
Author: Christina Karavasili
Author: Elisavet Elisavet Adamoudi
Author: Athina Bakopoulou
Author: Dimitrios G. Fatouros

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