Dual spindles assemble in bovine zygotes despite the presence of paternal centrosomes
Dual spindles assemble in bovine zygotes despite the presence of paternal centrosomes
The first mitosis of the mammalian embryo must partition the parental genomes contained in two pronuclei. In rodent zygotes, sperm centrosomes are degraded, and instead, acentriolar microtubule organizing centers and microtubule self-organization guide the assembly of two separate spindles around the genomes. In nonrodent mammals, including human or bovine, centrosomes are inherited from the sperm and have been widely assumed to be active. Whether nonrodent zygotes assemble a single centrosomal spindle around both genomes or follow the dual spindle self-assembly pathway is unclear. To address this, we investigated spindle assembly in bovine zygotes by systematic immunofluorescence and real-time light-sheet microscopy. We show that two independent spindles form despite the presence of centrosomes, which had little effect on spindle structure and were only loosely connected to the two spindles. We conclude that the dual spindle assembly pathway is conserved in nonrodent mammals. This could explain whole parental genome loss frequently observed in blastomeres of human IVF embryos.
Schneider, Isabell
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Ruijter-Villani, Marta de
6bb75247-b5ab-49e6-bae0-ec0ade6ac329
Hossain, M. Julius
bba1b875-7604-462b-a55b-ba0b54f728e8
Netherlands, Utrecht University Utrecht
413ebb52-6e2e-45b1-a463-6573a9980ed2
Ellenberg, Jan
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1 November 2021
Schneider, Isabell
4c31f717-963a-4e52-99f4-a0efb4d602b2
Ruijter-Villani, Marta de
6bb75247-b5ab-49e6-bae0-ec0ade6ac329
Hossain, M. Julius
bba1b875-7604-462b-a55b-ba0b54f728e8
Netherlands, Utrecht University Utrecht
413ebb52-6e2e-45b1-a463-6573a9980ed2
Ellenberg, Jan
7b8ab9a9-8076-4db1-b7a2-8445accc9b54
Schneider, Isabell, Ruijter-Villani, Marta de, Hossain, M. Julius, Netherlands, Utrecht University Utrecht and Ellenberg, Jan
(2021)
Dual spindles assemble in bovine zygotes despite the presence of paternal centrosomes.
Journal of Cell Biology, 220 (11).
(doi:10.1083/jcb.202010106).
Abstract
The first mitosis of the mammalian embryo must partition the parental genomes contained in two pronuclei. In rodent zygotes, sperm centrosomes are degraded, and instead, acentriolar microtubule organizing centers and microtubule self-organization guide the assembly of two separate spindles around the genomes. In nonrodent mammals, including human or bovine, centrosomes are inherited from the sperm and have been widely assumed to be active. Whether nonrodent zygotes assemble a single centrosomal spindle around both genomes or follow the dual spindle self-assembly pathway is unclear. To address this, we investigated spindle assembly in bovine zygotes by systematic immunofluorescence and real-time light-sheet microscopy. We show that two independent spindles form despite the presence of centrosomes, which had little effect on spindle structure and were only loosely connected to the two spindles. We conclude that the dual spindle assembly pathway is conserved in nonrodent mammals. This could explain whole parental genome loss frequently observed in blastomeres of human IVF embryos.
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jcb_202010106
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Accepted/In Press date: 2 September 2021
Published date: 1 November 2021
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Local EPrints ID: 456747
URI: http://eprints.soton.ac.uk/id/eprint/456747
ISSN: 1540-8140
PURE UUID: fefaeab8-77be-4ff0-a4e8-030ca23683c8
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Date deposited: 10 May 2022 16:46
Last modified: 16 Apr 2024 02:03
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Author:
Isabell Schneider
Author:
Marta de Ruijter-Villani
Author:
M. Julius Hossain
Author:
Utrecht University Utrecht Netherlands
Author:
Jan Ellenberg
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