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Microbe-immune crosstalk: Evidence that T Cells influence the development of the brain metabolome

Microbe-immune crosstalk: Evidence that T Cells influence the development of the brain metabolome
Microbe-immune crosstalk: Evidence that T Cells influence the development of the brain metabolome
Cross-talk between the immune system and the brain is essential to neuronal development, neuronal excitability, neuroplasticity, and neurotransmission. Gut microbiota are essential to immune system development and immune function; hence, it is essential to consider more broadly the microbiota-immune-brain axis in neurodevelopment. The gut, brain, and microbial metabolomes obtained from C57Bl/6 and T-cell-deficient mice across four developmental timepoints (postnatal day 17, 24, 28, and 84) were studied by 1H NMR spectroscopy. 16S rRNA gene sequencing was performed on cecal and fecal samples. In the absence of T-cells, the developmental trajectory of the gut microbiota and of the host's metabolic profile was altered. The novel insights from this work include (1) the requirement of functional T-cells for the normal trajectory of microbiotal development and the metabolic maturation of the supra-organism, (2) the potential role for Muribaculaceae taxa in modulating the cecal availability of metabolites previously implicated with a role in the gut-brain axis in T-cell deficient mice, and (3) the impact of T-cell-deficiency on central levels of neuroactive metabolites.
5-aminovalerate, Brain, Butyrate, Glucose-6-phosphate, Immunocompromised, Metabolome, Microbiota, Muribaculaceae, T cells, Valerate
1422-0067
Caspani, Giorgia
ca711d7a-4d4b-4b99-8550-db8ed3ad612e
Green, Miranda
89f979de-d07b-4ac1-affe-4611ac2f9eeb
Swann, Jonathan R
7c11a66b-f4b8-4dbf-aa17-ad8b0561b85c
Foster, Jane A
e1f25f28-2408-46bb-b5d7-4020b9e84c42
Caspani, Giorgia
ca711d7a-4d4b-4b99-8550-db8ed3ad612e
Green, Miranda
89f979de-d07b-4ac1-affe-4611ac2f9eeb
Swann, Jonathan R
7c11a66b-f4b8-4dbf-aa17-ad8b0561b85c
Foster, Jane A
e1f25f28-2408-46bb-b5d7-4020b9e84c42

Caspani, Giorgia, Green, Miranda, Swann, Jonathan R and Foster, Jane A (2022) Microbe-immune crosstalk: Evidence that T Cells influence the development of the brain metabolome. International Journal of Molecular Sciences, 23 (6), [3259]. (doi:10.3390/ijms23063259).

Record type: Article

Abstract

Cross-talk between the immune system and the brain is essential to neuronal development, neuronal excitability, neuroplasticity, and neurotransmission. Gut microbiota are essential to immune system development and immune function; hence, it is essential to consider more broadly the microbiota-immune-brain axis in neurodevelopment. The gut, brain, and microbial metabolomes obtained from C57Bl/6 and T-cell-deficient mice across four developmental timepoints (postnatal day 17, 24, 28, and 84) were studied by 1H NMR spectroscopy. 16S rRNA gene sequencing was performed on cecal and fecal samples. In the absence of T-cells, the developmental trajectory of the gut microbiota and of the host's metabolic profile was altered. The novel insights from this work include (1) the requirement of functional T-cells for the normal trajectory of microbiotal development and the metabolic maturation of the supra-organism, (2) the potential role for Muribaculaceae taxa in modulating the cecal availability of metabolites previously implicated with a role in the gut-brain axis in T-cell deficient mice, and (3) the impact of T-cell-deficiency on central levels of neuroactive metabolites.

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Accepted/In Press date: 10 March 2022
Published date: 17 March 2022
Additional Information: Funding Information: Funding: This work was supported by the Medical Research Council and National Institute for Health Research [grant number MC_PC_12025] and infrastructure support was provided by the National Institute for Health Research (NIHR) Imperial Biomedical Research Centre (BRC), and an operating grant from the Ontario Brain Institute (OBI–POND JAF). Giorgia Caspani was supported by the MRC (grant number MR/N014103/1). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.
Keywords: 5-aminovalerate, Brain, Butyrate, Glucose-6-phosphate, Immunocompromised, Metabolome, Microbiota, Muribaculaceae, T cells, Valerate

Identifiers

Local EPrints ID: 456763
URI: http://eprints.soton.ac.uk/id/eprint/456763
ISSN: 1422-0067
PURE UUID: 371b9b73-83e2-4d1c-aec6-95f211eca973
ORCID for Jonathan R Swann: ORCID iD orcid.org/0000-0002-6485-4529

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Date deposited: 10 May 2022 17:06
Last modified: 11 May 2022 02:00

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Contributors

Author: Giorgia Caspani
Author: Miranda Green
Author: Jane A Foster

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