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Modelling of filamentous phage-induced antibiotic tolerance of P. aeruginosa

Modelling of filamentous phage-induced antibiotic tolerance of P. aeruginosa
Modelling of filamentous phage-induced antibiotic tolerance of P. aeruginosa

Filamentous molecules tend to spontaneously assemble into liquid crystalline droplets with a tactoid morphology in environments with high concentration on non-adsorbing molecules. Tactoids of filamentous Pf bacteriophage, such as those produced by Pseudomonas aeruginosa, have been linked to increased antibiotic tolerance. We modelled this system and show that tactoids composed of filamentous Pf virions can lead to antibiotic tolerance by acting as an adsorptive diffusion barrier. The continuum model, reminiscent of descriptions of reactive diffusion in porous media, has been solved numerically and good agreement was found with the analytical results, obtained using a homogenisation approach. We find that the formation of tactoids significantly increases antibiotic diffusion times which may lead to stronger antibiotic resistance.

Anti-Bacterial Agents/pharmacology, Drug Resistance, Microbial, Drug Tolerance, Inovirus, Pseudomonas aeruginosa
1932-6203
e0261482
van Rossem, Maria
fc4152c5-324f-4849-a53a-c4591ff4b818
Wilks, Sandra
86c1f41a-12b3-451c-9245-b1a21775e993
Kaczmarek, Malgosia
408ec59b-8dba-41c1-89d0-af846d1bf327
Secor, Patrick R
d704b3f6-6489-47f6-8dc3-83aeea1fd8f5
D'Alessandro, Giampaolo
bad097e1-9506-4b6e-aa56-3e67a526e83b
van Rossem, Maria
fc4152c5-324f-4849-a53a-c4591ff4b818
Wilks, Sandra
86c1f41a-12b3-451c-9245-b1a21775e993
Kaczmarek, Malgosia
408ec59b-8dba-41c1-89d0-af846d1bf327
Secor, Patrick R
d704b3f6-6489-47f6-8dc3-83aeea1fd8f5
D'Alessandro, Giampaolo
bad097e1-9506-4b6e-aa56-3e67a526e83b

van Rossem, Maria, Wilks, Sandra, Kaczmarek, Malgosia, Secor, Patrick R and D'Alessandro, Giampaolo (2022) Modelling of filamentous phage-induced antibiotic tolerance of P. aeruginosa. PLoS ONE, 17 (4), e0261482, [e0261482]. (doi:10.1371/journal.pone.0261482).

Record type: Article

Abstract

Filamentous molecules tend to spontaneously assemble into liquid crystalline droplets with a tactoid morphology in environments with high concentration on non-adsorbing molecules. Tactoids of filamentous Pf bacteriophage, such as those produced by Pseudomonas aeruginosa, have been linked to increased antibiotic tolerance. We modelled this system and show that tactoids composed of filamentous Pf virions can lead to antibiotic tolerance by acting as an adsorptive diffusion barrier. The continuum model, reminiscent of descriptions of reactive diffusion in porous media, has been solved numerically and good agreement was found with the analytical results, obtained using a homogenisation approach. We find that the formation of tactoids significantly increases antibiotic diffusion times which may lead to stronger antibiotic resistance.

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Accepted/In Press date: 25 March 2022
Published date: 11 April 2022
Additional Information: Publisher Copyright: Copyright: © 2022 van Rossem et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.
Keywords: Anti-Bacterial Agents/pharmacology, Drug Resistance, Microbial, Drug Tolerance, Inovirus, Pseudomonas aeruginosa

Identifiers

Local EPrints ID: 457144
URI: http://eprints.soton.ac.uk/id/eprint/457144
ISSN: 1932-6203
PURE UUID: 9c47b334-9aab-4034-a46c-39749806149b
ORCID for Sandra Wilks: ORCID iD orcid.org/0000-0002-4134-9415
ORCID for Giampaolo D'Alessandro: ORCID iD orcid.org/0000-0001-9166-9356

Catalogue record

Date deposited: 24 May 2022 17:02
Last modified: 25 May 2022 01:35

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Contributors

Author: Maria van Rossem
Author: Sandra Wilks ORCID iD
Author: Patrick R Secor

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