Modelling of filamentous phage-induced antibiotic tolerance of P. aeruginosa
Modelling of filamentous phage-induced antibiotic tolerance of P. aeruginosa
Filamentous molecules tend to spontaneously assemble into liquid crystalline droplets with a tactoid morphology in environments with high concentration on non-adsorbing molecules. Tactoids of filamentous Pf bacteriophage, such as those produced by Pseudomonas aeruginosa, have been linked to increased antibiotic tolerance. We modelled this system and show that tactoids composed of filamentous Pf virions can lead to antibiotic tolerance by acting as an adsorptive diffusion barrier. The continuum model, reminiscent of descriptions of reactive diffusion in porous media, has been solved numerically and good agreement was found with the analytical results, obtained using a homogenisation approach. We find that the formation of tactoids significantly increases antibiotic diffusion times which may lead to stronger antibiotic resistance.
Anti-Bacterial Agents/pharmacology, Drug Resistance, Microbial, Drug Tolerance, Inovirus, Pseudomonas aeruginosa
e0261482
van Rossem, Maria
fc4152c5-324f-4849-a53a-c4591ff4b818
Wilks, Sandra
86c1f41a-12b3-451c-9245-b1a21775e993
Kaczmarek, Malgosia
408ec59b-8dba-41c1-89d0-af846d1bf327
Secor, Patrick R
d704b3f6-6489-47f6-8dc3-83aeea1fd8f5
D'Alessandro, Giampaolo
bad097e1-9506-4b6e-aa56-3e67a526e83b
11 April 2022
van Rossem, Maria
fc4152c5-324f-4849-a53a-c4591ff4b818
Wilks, Sandra
86c1f41a-12b3-451c-9245-b1a21775e993
Kaczmarek, Malgosia
408ec59b-8dba-41c1-89d0-af846d1bf327
Secor, Patrick R
d704b3f6-6489-47f6-8dc3-83aeea1fd8f5
D'Alessandro, Giampaolo
bad097e1-9506-4b6e-aa56-3e67a526e83b
van Rossem, Maria, Wilks, Sandra, Kaczmarek, Malgosia, Secor, Patrick R and D'Alessandro, Giampaolo
(2022)
Modelling of filamentous phage-induced antibiotic tolerance of P. aeruginosa.
PLoS ONE, 17 (4), , [e0261482].
(doi:10.1371/journal.pone.0261482).
Abstract
Filamentous molecules tend to spontaneously assemble into liquid crystalline droplets with a tactoid morphology in environments with high concentration on non-adsorbing molecules. Tactoids of filamentous Pf bacteriophage, such as those produced by Pseudomonas aeruginosa, have been linked to increased antibiotic tolerance. We modelled this system and show that tactoids composed of filamentous Pf virions can lead to antibiotic tolerance by acting as an adsorptive diffusion barrier. The continuum model, reminiscent of descriptions of reactive diffusion in porous media, has been solved numerically and good agreement was found with the analytical results, obtained using a homogenisation approach. We find that the formation of tactoids significantly increases antibiotic diffusion times which may lead to stronger antibiotic resistance.
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journal.pone.0261482
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Accepted/In Press date: 25 March 2022
Published date: 11 April 2022
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Copyright: © 2022 van Rossem et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Copyright 2022 Elsevier B.V., All rights reserved.
Keywords:
Anti-Bacterial Agents/pharmacology, Drug Resistance, Microbial, Drug Tolerance, Inovirus, Pseudomonas aeruginosa
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Local EPrints ID: 457144
URI: http://eprints.soton.ac.uk/id/eprint/457144
ISSN: 1932-6203
PURE UUID: 9c47b334-9aab-4034-a46c-39749806149b
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Date deposited: 24 May 2022 17:02
Last modified: 17 Mar 2024 02:45
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Author:
Maria van Rossem
Author:
Patrick R Secor
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