High-resolution genotyping of Lymphogranuloma Venereum (LGV) strains of Chlamydia trachomatis in London using multi-locus VNTR analysis-ompA genotyping (MLVA-ompA)
High-resolution genotyping of Lymphogranuloma Venereum (LGV) strains of Chlamydia trachomatis in London using multi-locus VNTR analysis-ompA genotyping (MLVA-ompA)
Background: lymphogranuloma venereum (LGV) is caused by Chlamydia trachomatis strains with ompA genotypes L1 to L3. An LGV epidemic associated with the L2b genotype has emerged in the past few decades amongst men who have sex with men (MSM). C. trachomatis genotypes can be discriminated by outer membrane protein A gene (ompA) sequencing, however this method has limited resolution. This study employed a high-resolution genotyping method, namely, multi-locus tandem repeat (VNTR) analysis with ompA sequencing (MLVA-ompA), to assess the distribution of LGV MLVA-ompA genotypes amongst individuals attending genitourinary medicine (GUM) clinics in London.
Methods: clinical specimens were collected from individuals attending eight London-based GUM clinics. Specimens that tested positive for C. trachomatis by commercial nucleic acid amplification test (NAAT) were confirmed as LGV by pmpH real-time PCR. LGV-positive DNA extracts were subsequently genotyped using MLVA-ompA.
Results: two hundred and thirty DNA extracts were confirmed as LGV, and 162 (70%) yielded complete MLVA-ompA genotypes. Six LGV MLVA-ompA genotypes were identified: 1.9.2b-L2, 1.9.3b-L2b, 1.9.2b-L2b, 1.9.2b-L2b/D, 1.4a.2b-L2b, and 5.9.2b-L1. The following LGV ompA genotypes were identified (in descending order of abundance): L2, L2b, L2b/D, and L1. Eight ompA sequences with the hybrid L2b/D profile were detected. The hybrid sequence was identical to the ompA of a recombinant L2b/D strain detected in Portugal in 2017.
Conclusions: the L2 ompA genotype was found to predominate in the London study population. The study detected an unusual hybrid L2b/D ompA profile that was previously reported in Portugal. We recommend further monitoring and surveillance of LGV strains within the UK population.
Adult, Bacterial Outer Membrane Proteins, Chlamydia trachomatis, Genotype, Homosexuality, Male, Humans, Lymphogranuloma Venereum/epidemiology, Male
e0254233
Manning, Chloe
de04ad9e-1241-4dd4-819e-55db411c63d2
O'Neill, Colette
3de0c221-6578-4a1a-96bd-2a3fba2b6193
Clarke, Ian N
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Rebec, Monica
40716d12-b431-4c94-a456-c9a86850a4e4
Cliff, Penelope R
a9eae12a-8354-4623-b9b2-da3e10c179ad
Marsh, Peter
2f77a131-1871-45b8-a5d8-a5ea9beb430a
8 July 2021
Manning, Chloe
de04ad9e-1241-4dd4-819e-55db411c63d2
O'Neill, Colette
3de0c221-6578-4a1a-96bd-2a3fba2b6193
Clarke, Ian N
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Rebec, Monica
40716d12-b431-4c94-a456-c9a86850a4e4
Cliff, Penelope R
a9eae12a-8354-4623-b9b2-da3e10c179ad
Marsh, Peter
2f77a131-1871-45b8-a5d8-a5ea9beb430a
Manning, Chloe, O'Neill, Colette, Clarke, Ian N, Rebec, Monica, Cliff, Penelope R and Marsh, Peter
(2021)
High-resolution genotyping of Lymphogranuloma Venereum (LGV) strains of Chlamydia trachomatis in London using multi-locus VNTR analysis-ompA genotyping (MLVA-ompA).
PLoS ONE, 16 (7), .
(doi:10.1371/journal.pone.0254233).
Abstract
Background: lymphogranuloma venereum (LGV) is caused by Chlamydia trachomatis strains with ompA genotypes L1 to L3. An LGV epidemic associated with the L2b genotype has emerged in the past few decades amongst men who have sex with men (MSM). C. trachomatis genotypes can be discriminated by outer membrane protein A gene (ompA) sequencing, however this method has limited resolution. This study employed a high-resolution genotyping method, namely, multi-locus tandem repeat (VNTR) analysis with ompA sequencing (MLVA-ompA), to assess the distribution of LGV MLVA-ompA genotypes amongst individuals attending genitourinary medicine (GUM) clinics in London.
Methods: clinical specimens were collected from individuals attending eight London-based GUM clinics. Specimens that tested positive for C. trachomatis by commercial nucleic acid amplification test (NAAT) were confirmed as LGV by pmpH real-time PCR. LGV-positive DNA extracts were subsequently genotyped using MLVA-ompA.
Results: two hundred and thirty DNA extracts were confirmed as LGV, and 162 (70%) yielded complete MLVA-ompA genotypes. Six LGV MLVA-ompA genotypes were identified: 1.9.2b-L2, 1.9.3b-L2b, 1.9.2b-L2b, 1.9.2b-L2b/D, 1.4a.2b-L2b, and 5.9.2b-L1. The following LGV ompA genotypes were identified (in descending order of abundance): L2, L2b, L2b/D, and L1. Eight ompA sequences with the hybrid L2b/D profile were detected. The hybrid sequence was identical to the ompA of a recombinant L2b/D strain detected in Portugal in 2017.
Conclusions: the L2 ompA genotype was found to predominate in the London study population. The study detected an unusual hybrid L2b/D ompA profile that was previously reported in Portugal. We recommend further monitoring and surveillance of LGV strains within the UK population.
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journal.pone.0254233 (1)
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Accepted/In Press date: 22 June 2021
Published date: 8 July 2021
Keywords:
Adult, Bacterial Outer Membrane Proteins, Chlamydia trachomatis, Genotype, Homosexuality, Male, Humans, Lymphogranuloma Venereum/epidemiology, Male
Identifiers
Local EPrints ID: 457204
URI: http://eprints.soton.ac.uk/id/eprint/457204
ISSN: 1932-6203
PURE UUID: 8b500ff3-f13e-4535-b3b1-ad058d6b28b8
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Date deposited: 26 May 2022 16:41
Last modified: 17 Mar 2024 02:32
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Author:
Chloe Manning
Author:
Colette O'Neill
Author:
Monica Rebec
Author:
Penelope R Cliff
Author:
Peter Marsh
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