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Electrospun nanofiber films suppress inflammation in vitro and eradicate endodontic bacterial infection in an E. faecalis-infected Ex Vivo Human Tooth Culture Model

Electrospun nanofiber films suppress inflammation in vitro and eradicate endodontic bacterial infection in an E. faecalis-infected Ex Vivo Human Tooth Culture Model
Electrospun nanofiber films suppress inflammation in vitro and eradicate endodontic bacterial infection in an E. faecalis-infected Ex Vivo Human Tooth Culture Model
Treatment failure of endodontic infections and their concurrent inflammations is commonly associated with microbial persistence and reinfection, also stemming from the anatomical restrictions of the root canal system. Aiming to address the shortcomings of current treatment options, a fast-disintegrating nanofibrous film was developed for the intracanal coadministration of an antimicrobial (ZnO nanoparticles) and an anti-inflammatory (ketoprofen) agent. The electrospun films were fabricated based on polymers that dissolve rapidly to constitute the actives readily available at the site of action, aiming to eliminate both microbial infection and inflammation. The anti-inflammatory potency of the nanofiber films was assessed in an in vitro model of lipopolysaccharide (LPS)-stimulated RAW 264.7 cells after confirming their biocompatibility in the same cell line. The nanofiber films were found effective against Enterococcus faecalis, one of the most prominent pathogens inside the root canal space, both in vitro and ex vivo using a human tooth model experimentally infected with E. faecalis. The physical properties and antibacterial and anti-inflammatory potency of the proposed electrospun nanofiber films constitute a promising therapeutic module in the endodontic therapy of nonvital infected teeth. All manuscripts must be accompanied by an abstract. The abstract should briefly state the problem or purpose of the research, indicate the theoretical or experimental plan used, summarize the principal findings, and point out major conclusions.
Anti-Bacterial Agents/pharmacology, Anti-Infective Agents, Bacterial Infections, Enterococcus faecalis, Humans, Inflammation/drug therapy, Nanofibers/therapeutic use
2373-9878
2096-2110
Chachlioutaki, Konstantina
aa15e449-9ebc-4a23-a5a3-4a0a1327fc8e
Karavasili, Christina
f174d197-8ba7-4765-adc8-99fa2f1c86fa
Adamoudi, Elisavet
1a092bc0-bb61-4839-80f5-261b9f079b74
Tsitsos, Anestis
ac28e173-72d1-4aaf-b73c-8a9ee40428d2
Economou, Vangelis
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Beltes, Charis
6d2e27f7-3f05-4e74-91c4-13252cd32a96
Bouropoulos, Nikolaos
fadadea1-2aa2-4cd9-a7e5-33ba790cd45f
Katsamenis, Orestis L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Doherty, Regan
da82ae44-70e7-41d4-9150-6ab3ce459738
Bakopoulou, Athina
c0e871af-bc82-4232-ae1d-18429e80c737
Fatouros, Dimitrios G.
4a716c96-a8ba-4fbf-b3db-3b2a3a2794c4
Chachlioutaki, Konstantina
aa15e449-9ebc-4a23-a5a3-4a0a1327fc8e
Karavasili, Christina
f174d197-8ba7-4765-adc8-99fa2f1c86fa
Adamoudi, Elisavet
1a092bc0-bb61-4839-80f5-261b9f079b74
Tsitsos, Anestis
ac28e173-72d1-4aaf-b73c-8a9ee40428d2
Economou, Vangelis
2a0f0d95-9004-4111-8f0f-d67d0ef1f91f
Beltes, Charis
6d2e27f7-3f05-4e74-91c4-13252cd32a96
Bouropoulos, Nikolaos
fadadea1-2aa2-4cd9-a7e5-33ba790cd45f
Katsamenis, Orestis L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Doherty, Regan
da82ae44-70e7-41d4-9150-6ab3ce459738
Bakopoulou, Athina
c0e871af-bc82-4232-ae1d-18429e80c737
Fatouros, Dimitrios G.
4a716c96-a8ba-4fbf-b3db-3b2a3a2794c4

Chachlioutaki, Konstantina, Karavasili, Christina, Adamoudi, Elisavet, Tsitsos, Anestis, Economou, Vangelis, Beltes, Charis, Bouropoulos, Nikolaos, Katsamenis, Orestis L., Doherty, Regan, Bakopoulou, Athina and Fatouros, Dimitrios G. (2022) Electrospun nanofiber films suppress inflammation in vitro and eradicate endodontic bacterial infection in an E. faecalis-infected Ex Vivo Human Tooth Culture Model. ACS Biomaterials Science and Engineering, 8 (5), 2096-2110. (doi:10.1021/acsbiomaterials.2c00150).

Record type: Article

Abstract

Treatment failure of endodontic infections and their concurrent inflammations is commonly associated with microbial persistence and reinfection, also stemming from the anatomical restrictions of the root canal system. Aiming to address the shortcomings of current treatment options, a fast-disintegrating nanofibrous film was developed for the intracanal coadministration of an antimicrobial (ZnO nanoparticles) and an anti-inflammatory (ketoprofen) agent. The electrospun films were fabricated based on polymers that dissolve rapidly to constitute the actives readily available at the site of action, aiming to eliminate both microbial infection and inflammation. The anti-inflammatory potency of the nanofiber films was assessed in an in vitro model of lipopolysaccharide (LPS)-stimulated RAW 264.7 cells after confirming their biocompatibility in the same cell line. The nanofiber films were found effective against Enterococcus faecalis, one of the most prominent pathogens inside the root canal space, both in vitro and ex vivo using a human tooth model experimentally infected with E. faecalis. The physical properties and antibacterial and anti-inflammatory potency of the proposed electrospun nanofiber films constitute a promising therapeutic module in the endodontic therapy of nonvital infected teeth. All manuscripts must be accompanied by an abstract. The abstract should briefly state the problem or purpose of the research, indicate the theoretical or experimental plan used, summarize the principal findings, and point out major conclusions.

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Accepted/In Press date: 6 April 2022
e-pub ahead of print date: 15 April 2022
Published date: 9 May 2022
Additional Information: Funding Information: Part of this work was supported by the Wellcome Trust Biomedical Resource and Technology Development Grant 212940/Z/18/Z and the National Research Facility for Lab X-ray CT (NXCT) through EPSRC grant EP/T02593X/1. Publisher Copyright: © 2022 American Chemical Society. All rights reserved. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.
Keywords: Anti-Bacterial Agents/pharmacology, Anti-Infective Agents, Bacterial Infections, Enterococcus faecalis, Humans, Inflammation/drug therapy, Nanofibers/therapeutic use

Identifiers

Local EPrints ID: 457456
URI: http://eprints.soton.ac.uk/id/eprint/457456
ISSN: 2373-9878
PURE UUID: 2d486f61-f537-4e2e-9a49-cc00481396ac
ORCID for Orestis L. Katsamenis: ORCID iD orcid.org/0000-0003-4367-4147

Catalogue record

Date deposited: 09 Jun 2022 16:32
Last modified: 19 Oct 2022 01:43

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Contributors

Author: Konstantina Chachlioutaki
Author: Christina Karavasili
Author: Elisavet Adamoudi
Author: Anestis Tsitsos
Author: Vangelis Economou
Author: Charis Beltes
Author: Nikolaos Bouropoulos
Author: Regan Doherty
Author: Athina Bakopoulou
Author: Dimitrios G. Fatouros

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