The University of Southampton
University of Southampton Institutional Repository

Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites

Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites
Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites
The eukaryotic phylum Apicomplexa encompasses thousands of obligate intracellular parasites of humans and animals with immense socio-economic and health impacts. We sequenced nuclear genomes of Chromera velia and Vitrella brassicaformis, free-living non-parasitic photosynthetic algae closely related to apicomplexans. Proteins from key metabolic pathways and from the endomembrane trafficking systems associated with a free-living lifestyle have been progressively and non-randomly lost during adaptation to parasitism. The free-living ancestor contained a broad repertoire of genes many of which were repurposed for parasitic processes, such as extracellular proteins, components of a motility apparatus, and DNA- and RNA-binding protein families. Based on transcriptome analyses across 36 environmental conditions, Chromera orthologs of apicomplexan invasion-related motility genes were co-regulated with genes encoding the flagellar apparatus, supporting the functional contribution of flagella to the evolution of invasion machinery. This study provides insights into how obligate parasites with diverse life strategies arose from a once free-living phototrophic marine alga.
2050-084X
Woo, Yong H.
4157f7d5-ebc4-4f81-a4ee-fdfd864caf88
Ansari, Hifzur
8d2f3746-cd6d-4198-ae0f-e11e743bae5a
Otto, Thomas D.
db9ecc67-9454-4c89-b8d4-c6ac662a16b2
Klinger, Christen M.
267741c7-84a4-4b50-af00-859bf7e9c7dc
Kolisko, Martin
a97db0e1-58b2-4695-9668-f5596b7e4175
Michalek, Jan
3b5f3fba-2982-40c1-8e73-da8abf7fcafd
Saxena, Alka
8647cce2-f86b-4046-bba1-ed4ccdae32c5
Shanmugam, Dhanasekaran
23c9b0d3-1cea-454c-9d75-8e4e3fe28d42
Tayyrov, Annageldi
5514f6ef-0208-4ec1-86ec-a11b7b5b8c65
Veluchamy, Alaguraj
121d1f44-a2d2-47b4-ac6e-63c7fdaca7f4
Ali, Shahjahan
d74a8d42-6179-425c-bb16-efc5625ae47a
Bernal, Axel
1ab224c2-c257-42ef-a256-e81149d2d35e
del Campo, Javier
048039b0-75e1-4063-8b49-c137ede13345
Cihlar, Jaromir
5b033513-7e64-4cdd-8704-187e1c7ae5d3
Flegontov, Pavel
5d93825a-a2ec-4545-ac52-3099bf68836e
Gornik, Sebastian G.
9dba3c0a-d6b6-419a-b2ff-514d5f6b9240
Hajduskova, Eva
903228e9-7b56-4360-8a00-b0b36e169989
Horak, Ales
bf69eb70-a1bb-4961-8d1b-dc3699608154
Janouskovec, Jan
fbaa4a5d-872e-465b-b2c3-bb35df455cc6
Katris, Nicholas J.
865d88e2-d635-4d0a-bab8-d891eb2d4e0b
Mast, Fred D.
7ed64e8b-a7eb-4104-8121-b2234dbab47e
Miranda-Saavedra, Diego
39b175cb-c5dc-4a35-8bf8-216dab5db4d8
Mourier, Tobias
748bf0dc-0b94-425e-8ecd-8aea051d0d0c
Naeem, Raeece
750be556-66c8-4f77-ab2c-d336ee9ef33a
Nair, Mridul
0a2c4201-deac-4754-9811-6fdf6be2315c
Panigrahi, Aswini K.
bb578ea6-fe83-4742-a082-ce1063f224bd
Rawlings, Neil D.
f0805db3-8b8a-4722-9846-cfc65b64a0a3
Padron-Regalado, Eriko
ffed4d1b-fe08-4013-bc6e-ca3bcc1f492c
Ramaprasad, Abhinay
c1ed4d6b-da5d-46b6-bc6e-cabd2eb0ec5d
Samad, Nadira
c79f68ff-f188-4d72-a2d9-d63356cd9ddc
Tomcala, Ales
57d14e1a-6973-49ef-9754-7a78e6aa3d2b
Wilkes, Jon
639425fd-8d9f-4fc8-8fd1-4c648fe15149
Neafsey, Daniel E.
205cbd4a-8544-4940-903b-1bd2d4079423
Doerig, Christian
342e8c4c-6e6b-4364-8352-d49d445e0789
Bowler, Chris
138c3382-d258-4ef3-8f5f-e16603c21880
Keeling, Patrick J.
fd51c2ef-1daa-442d-b186-71001aa7ca7d
Roos, David S.
f7b4899a-7811-431d-a73d-6bd08900d53f
Dacks, Joel B.
6b07ea4e-af69-4d7d-9def-f5ff1323055d
Templeton, Thomas J.
221d684a-c886-4dc4-b517-f6ed8472e57b
Waller, Ross F.
819d6fe6-79c8-4cee-aaf4-bacdd6c1d1c6
Lukes, Julius
4fe15ca8-205c-4a69-8e67-464947ea25ed
Obornik, Miroslav
0d0adb21-b7c9-41e4-a0dc-87fa6a3a7b89
Pain, Arnab
83778ee4-788e-402f-a4a6-7fe79222c921
Woo, Yong H.
4157f7d5-ebc4-4f81-a4ee-fdfd864caf88
Ansari, Hifzur
8d2f3746-cd6d-4198-ae0f-e11e743bae5a
Otto, Thomas D.
db9ecc67-9454-4c89-b8d4-c6ac662a16b2
Klinger, Christen M.
267741c7-84a4-4b50-af00-859bf7e9c7dc
Kolisko, Martin
a97db0e1-58b2-4695-9668-f5596b7e4175
Michalek, Jan
3b5f3fba-2982-40c1-8e73-da8abf7fcafd
Saxena, Alka
8647cce2-f86b-4046-bba1-ed4ccdae32c5
Shanmugam, Dhanasekaran
23c9b0d3-1cea-454c-9d75-8e4e3fe28d42
Tayyrov, Annageldi
5514f6ef-0208-4ec1-86ec-a11b7b5b8c65
Veluchamy, Alaguraj
121d1f44-a2d2-47b4-ac6e-63c7fdaca7f4
Ali, Shahjahan
d74a8d42-6179-425c-bb16-efc5625ae47a
Bernal, Axel
1ab224c2-c257-42ef-a256-e81149d2d35e
del Campo, Javier
048039b0-75e1-4063-8b49-c137ede13345
Cihlar, Jaromir
5b033513-7e64-4cdd-8704-187e1c7ae5d3
Flegontov, Pavel
5d93825a-a2ec-4545-ac52-3099bf68836e
Gornik, Sebastian G.
9dba3c0a-d6b6-419a-b2ff-514d5f6b9240
Hajduskova, Eva
903228e9-7b56-4360-8a00-b0b36e169989
Horak, Ales
bf69eb70-a1bb-4961-8d1b-dc3699608154
Janouskovec, Jan
fbaa4a5d-872e-465b-b2c3-bb35df455cc6
Katris, Nicholas J.
865d88e2-d635-4d0a-bab8-d891eb2d4e0b
Mast, Fred D.
7ed64e8b-a7eb-4104-8121-b2234dbab47e
Miranda-Saavedra, Diego
39b175cb-c5dc-4a35-8bf8-216dab5db4d8
Mourier, Tobias
748bf0dc-0b94-425e-8ecd-8aea051d0d0c
Naeem, Raeece
750be556-66c8-4f77-ab2c-d336ee9ef33a
Nair, Mridul
0a2c4201-deac-4754-9811-6fdf6be2315c
Panigrahi, Aswini K.
bb578ea6-fe83-4742-a082-ce1063f224bd
Rawlings, Neil D.
f0805db3-8b8a-4722-9846-cfc65b64a0a3
Padron-Regalado, Eriko
ffed4d1b-fe08-4013-bc6e-ca3bcc1f492c
Ramaprasad, Abhinay
c1ed4d6b-da5d-46b6-bc6e-cabd2eb0ec5d
Samad, Nadira
c79f68ff-f188-4d72-a2d9-d63356cd9ddc
Tomcala, Ales
57d14e1a-6973-49ef-9754-7a78e6aa3d2b
Wilkes, Jon
639425fd-8d9f-4fc8-8fd1-4c648fe15149
Neafsey, Daniel E.
205cbd4a-8544-4940-903b-1bd2d4079423
Doerig, Christian
342e8c4c-6e6b-4364-8352-d49d445e0789
Bowler, Chris
138c3382-d258-4ef3-8f5f-e16603c21880
Keeling, Patrick J.
fd51c2ef-1daa-442d-b186-71001aa7ca7d
Roos, David S.
f7b4899a-7811-431d-a73d-6bd08900d53f
Dacks, Joel B.
6b07ea4e-af69-4d7d-9def-f5ff1323055d
Templeton, Thomas J.
221d684a-c886-4dc4-b517-f6ed8472e57b
Waller, Ross F.
819d6fe6-79c8-4cee-aaf4-bacdd6c1d1c6
Lukes, Julius
4fe15ca8-205c-4a69-8e67-464947ea25ed
Obornik, Miroslav
0d0adb21-b7c9-41e4-a0dc-87fa6a3a7b89
Pain, Arnab
83778ee4-788e-402f-a4a6-7fe79222c921

Woo, Yong H., Ansari, Hifzur, Otto, Thomas D., Klinger, Christen M., Kolisko, Martin, Michalek, Jan, Saxena, Alka, Shanmugam, Dhanasekaran, Tayyrov, Annageldi, Veluchamy, Alaguraj, Ali, Shahjahan, Bernal, Axel, del Campo, Javier, Cihlar, Jaromir, Flegontov, Pavel, Gornik, Sebastian G., Hajduskova, Eva, Horak, Ales, Janouskovec, Jan, Katris, Nicholas J., Mast, Fred D., Miranda-Saavedra, Diego, Mourier, Tobias, Naeem, Raeece, Nair, Mridul, Panigrahi, Aswini K., Rawlings, Neil D., Padron-Regalado, Eriko, Ramaprasad, Abhinay, Samad, Nadira, Tomcala, Ales, Wilkes, Jon, Neafsey, Daniel E., Doerig, Christian, Bowler, Chris, Keeling, Patrick J., Roos, David S., Dacks, Joel B., Templeton, Thomas J., Waller, Ross F., Lukes, Julius, Obornik, Miroslav and Pain, Arnab (2015) Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites. eLife. (doi:10.7554/ELIFE.06974).

Record type: Article

Abstract

The eukaryotic phylum Apicomplexa encompasses thousands of obligate intracellular parasites of humans and animals with immense socio-economic and health impacts. We sequenced nuclear genomes of Chromera velia and Vitrella brassicaformis, free-living non-parasitic photosynthetic algae closely related to apicomplexans. Proteins from key metabolic pathways and from the endomembrane trafficking systems associated with a free-living lifestyle have been progressively and non-randomly lost during adaptation to parasitism. The free-living ancestor contained a broad repertoire of genes many of which were repurposed for parasitic processes, such as extracellular proteins, components of a motility apparatus, and DNA- and RNA-binding protein families. Based on transcriptome analyses across 36 environmental conditions, Chromera orthologs of apicomplexan invasion-related motility genes were co-regulated with genes encoding the flagellar apparatus, supporting the functional contribution of flagella to the evolution of invasion machinery. This study provides insights into how obligate parasites with diverse life strategies arose from a once free-living phototrophic marine alga.

This record has no associated files available for download.

More information

Published date: 15 July 2015

Identifiers

Local EPrints ID: 457550
URI: http://eprints.soton.ac.uk/id/eprint/457550
ISSN: 2050-084X
PURE UUID: df5e5da9-af19-47a4-8030-3ac8aeb53a8b
ORCID for Jan Janouskovec: ORCID iD orcid.org/0000-0001-6547-749X

Catalogue record

Date deposited: 13 Jun 2022 16:34
Last modified: 17 Mar 2024 04:11

Export record

Altmetrics

Contributors

Author: Yong H. Woo
Author: Hifzur Ansari
Author: Thomas D. Otto
Author: Christen M. Klinger
Author: Martin Kolisko
Author: Jan Michalek
Author: Alka Saxena
Author: Dhanasekaran Shanmugam
Author: Annageldi Tayyrov
Author: Alaguraj Veluchamy
Author: Shahjahan Ali
Author: Axel Bernal
Author: Javier del Campo
Author: Jaromir Cihlar
Author: Pavel Flegontov
Author: Sebastian G. Gornik
Author: Eva Hajduskova
Author: Ales Horak
Author: Jan Janouskovec ORCID iD
Author: Nicholas J. Katris
Author: Fred D. Mast
Author: Diego Miranda-Saavedra
Author: Tobias Mourier
Author: Raeece Naeem
Author: Mridul Nair
Author: Aswini K. Panigrahi
Author: Neil D. Rawlings
Author: Eriko Padron-Regalado
Author: Abhinay Ramaprasad
Author: Nadira Samad
Author: Ales Tomcala
Author: Jon Wilkes
Author: Daniel E. Neafsey
Author: Christian Doerig
Author: Chris Bowler
Author: Patrick J. Keeling
Author: David S. Roos
Author: Joel B. Dacks
Author: Thomas J. Templeton
Author: Ross F. Waller
Author: Julius Lukes
Author: Miroslav Obornik
Author: Arnab Pain

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×