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Uncovering microbial populations in the lumen of neonatal enteral feeding tubes utilising 16s rRNA sequencing

Uncovering microbial populations in the lumen of neonatal enteral feeding tubes utilising 16s rRNA sequencing
Uncovering microbial populations in the lumen of neonatal enteral feeding tubes utilising 16s rRNA sequencing
Gastrointestinal microbiome is increasingly implicated in the morbidity associated with being born preterm. Enteral tubes (ET) are essential for the nutritional care of preterm infants. Limited culture-based studies have suggested they are colonised by high densities of microorganisms. Microbial DNA was extracted from 60 ETs retrieved from infants in a tertiary neonatal unit and analysed by16s rRNA sequencing of the V4 variable region. Relative abundance analysis on dominant microorganisms demonstrated that compared to breast milk, formula significantly increased abundance of Streptococcus spp and significantly decreased Enterococcus spp and Enterobacteriaceae Vaginal birth was also associated with significantly increased relative abundance of Streptococcus . This study more accurately demonstrates the extent of microbial diversity in neonatal ETs, with feeding regime significantly influencing colonisation patterns. Colonisation with unwanted organisms, as a result of specific care regimes, could result in disruption of the fragile infant gut microbiome, with implications for long-term morbidity.
Winnard, CJ
23a6fa57-b2b3-465c-9fda-9b358d54bddd
Green, S
249b93db-c7c7-4c89-bc6d-ded88bcb15ea
Baylay, A
08ea69f8-9910-4c8b-86a9-602e45b8a44e
Johnson, MJ
f2008e92-3c20-495b-94ce-1debe695d6dc
Fader, M
c318f942-2ddb-462a-9183-8b678faf7277
Keevil, CW
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Wilks, S
86c1f41a-12b3-451c-9245-b1a21775e993
Winnard, CJ
23a6fa57-b2b3-465c-9fda-9b358d54bddd
Green, S
249b93db-c7c7-4c89-bc6d-ded88bcb15ea
Baylay, A
08ea69f8-9910-4c8b-86a9-602e45b8a44e
Johnson, MJ
f2008e92-3c20-495b-94ce-1debe695d6dc
Fader, M
c318f942-2ddb-462a-9183-8b678faf7277
Keevil, CW
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Wilks, S
86c1f41a-12b3-451c-9245-b1a21775e993

[Unknown type: UNSPECIFIED]

Record type: UNSPECIFIED

Abstract

Gastrointestinal microbiome is increasingly implicated in the morbidity associated with being born preterm. Enteral tubes (ET) are essential for the nutritional care of preterm infants. Limited culture-based studies have suggested they are colonised by high densities of microorganisms. Microbial DNA was extracted from 60 ETs retrieved from infants in a tertiary neonatal unit and analysed by16s rRNA sequencing of the V4 variable region. Relative abundance analysis on dominant microorganisms demonstrated that compared to breast milk, formula significantly increased abundance of Streptococcus spp and significantly decreased Enterococcus spp and Enterobacteriaceae Vaginal birth was also associated with significantly increased relative abundance of Streptococcus . This study more accurately demonstrates the extent of microbial diversity in neonatal ETs, with feeding regime significantly influencing colonisation patterns. Colonisation with unwanted organisms, as a result of specific care regimes, could result in disruption of the fragile infant gut microbiome, with implications for long-term morbidity.

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e-pub ahead of print date: 3 March 2020
Additional Information: The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

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Local EPrints ID: 458006
URI: http://eprints.soton.ac.uk/id/eprint/458006
PURE UUID: 6e7e89ff-7eb3-485e-bdb0-35319fa9f35f
ORCID for A Baylay: ORCID iD orcid.org/0000-0002-0814-0740
ORCID for CW Keevil: ORCID iD orcid.org/0000-0003-1917-7706
ORCID for S Wilks: ORCID iD orcid.org/0000-0002-4134-9415

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Date deposited: 24 Jun 2022 17:28
Last modified: 25 Jun 2022 01:47

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Contributors

Author: CJ Winnard
Author: S Green
Author: A Baylay ORCID iD
Author: MJ Johnson
Author: M Fader
Author: CW Keevil ORCID iD
Author: S Wilks ORCID iD

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