Uncovering microbial populations in the lumen of neonatal enteral feeding tubes utilising 16s rRNA sequencing
Uncovering microbial populations in the lumen of neonatal enteral feeding tubes utilising 16s rRNA sequencing
Gastrointestinal microbiome is increasingly implicated in the morbidity associated with being born preterm. Enteral tubes (ET) are essential for the nutritional care of preterm infants. Limited culture-based studies have suggested they are colonised by high densities of microorganisms. Microbial DNA was extracted from 60 ETs retrieved from infants in a tertiary neonatal unit and analysed by16s rRNA sequencing of the V4 variable region. Relative abundance analysis on dominant microorganisms demonstrated that compared to breast milk, formula significantly increased abundance of Streptococcus spp and significantly decreased Enterococcus spp and Enterobacteriaceae Vaginal birth was also associated with significantly increased relative abundance of Streptococcus . This study more accurately demonstrates the extent of microbial diversity in neonatal ETs, with feeding regime significantly influencing colonisation patterns. Colonisation with unwanted organisms, as a result of specific care regimes, could result in disruption of the fragile infant gut microbiome, with implications for long-term morbidity.
Winnard, CJ
23a6fa57-b2b3-465c-9fda-9b358d54bddd
Green, S
249b93db-c7c7-4c89-bc6d-ded88bcb15ea
Baylay, A
08ea69f8-9910-4c8b-86a9-602e45b8a44e
Johnson, MJ
f2008e92-3c20-495b-94ce-1debe695d6dc
Fader, M
c318f942-2ddb-462a-9183-8b678faf7277
Keevil, CW
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Wilks, S
86c1f41a-12b3-451c-9245-b1a21775e993
Winnard, CJ
23a6fa57-b2b3-465c-9fda-9b358d54bddd
Green, S
249b93db-c7c7-4c89-bc6d-ded88bcb15ea
Baylay, A
08ea69f8-9910-4c8b-86a9-602e45b8a44e
Johnson, MJ
f2008e92-3c20-495b-94ce-1debe695d6dc
Fader, M
c318f942-2ddb-462a-9183-8b678faf7277
Keevil, CW
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Wilks, S
86c1f41a-12b3-451c-9245-b1a21775e993
[Unknown type: UNSPECIFIED]
Abstract
Gastrointestinal microbiome is increasingly implicated in the morbidity associated with being born preterm. Enteral tubes (ET) are essential for the nutritional care of preterm infants. Limited culture-based studies have suggested they are colonised by high densities of microorganisms. Microbial DNA was extracted from 60 ETs retrieved from infants in a tertiary neonatal unit and analysed by16s rRNA sequencing of the V4 variable region. Relative abundance analysis on dominant microorganisms demonstrated that compared to breast milk, formula significantly increased abundance of Streptococcus spp and significantly decreased Enterococcus spp and Enterobacteriaceae Vaginal birth was also associated with significantly increased relative abundance of Streptococcus . This study more accurately demonstrates the extent of microbial diversity in neonatal ETs, with feeding regime significantly influencing colonisation patterns. Colonisation with unwanted organisms, as a result of specific care regimes, could result in disruption of the fragile infant gut microbiome, with implications for long-term morbidity.
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e-pub ahead of print date: 3 March 2020
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Local EPrints ID: 458006
URI: http://eprints.soton.ac.uk/id/eprint/458006
PURE UUID: 6e7e89ff-7eb3-485e-bdb0-35319fa9f35f
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Date deposited: 24 Jun 2022 17:28
Last modified: 17 Mar 2024 02:54
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Author:
CJ Winnard
Author:
S Green
Author:
MJ Johnson
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