READ ME File For 'Dataset for Single-frame 3D lensless imaging of pollen grains via deep learning'

Dataset DOI: 10.5258/SOTON/D2113

ReadMe Author: James A Grant-Jacob  University of Southampton https://orcid.org/0000-0002-4270-4247

This dataset supports the publication:
AUTHORS:James A. Grant-Jacob, Matthew Praeger, Robert W. Eason and Ben Mills
TITLE:Single-frame 3D lensless imaging of pollen grains via deep learning
JOURNAL: Optics Express
PAPER DOI IF KNOWN: 


This dataset contains:

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Table_1.txt Data for Volume and SSIM for the 2 genreated pollen grains. 

The figures are as follows:

Figure 1. Diagram showing the experimental setup, which includes a laser beam that was focussed onto pollen grains present on a glass slide. The light scattered in the forward direction from the pollen grains was collected by a camera sensor placed ~ 1 mm away from the glass slide. The pollen grains were imaged via back-reflection of white light onto a separate camera sensor. (b) Antirrhinum majus experimental scattering pattern and (c) corresponding maximum intensity projection microscope image.

Figure 2. Examples of (a) Antirrhinum majus, (b) Populus deltoides and (c) Narcissus pollen grains, showing the experimental scattering pattern (top) with corresponding maximum intensity projection image (middle) and 3D image of pollen in 128 × 128 × 128-pixel volume (bottom). Here, each XYZ pixel in the 3D volume is ~ 0.5 µm in length.

Figure 3. Examples of the experimental scattering pattern with a colour gradient in the green and blue channels (GB), indicating different heights for Z-axis information related to the Z-stack images from the corresponding 3D image of a Populus deltoides pollen grain.

Figure 4. Simplified diagram of the neural network. 

Figure 5. The capability of the image neural network for (a) Populus deltoides and (b) Antirrhinum majus. In each case a comparison between actual experiment images (1st row) and generated images (2nd row) of the pollen grains is provided. From left to right, the columns represent a 3D view (1st column), X-Y plane (2nd column) and X-Z plane (3rd column). Here, 1 pixel is ~ 0.5 µm in length. The maximum sizes of the pollen grains in X, Y and Z are indicated by the dashed lines and arrows. 

Figure 6. Generated 3D images of pollen using masked scatterings patterns and corresponding graphs of total volume and L1 comparison (sum of the absolute difference between volume of unmasked generated and volume of masked generated) for (a) Populus deltoides and (b) Antirrhinum majus, where (i-iii) are volumes for different mask radii, indicated in the graphs. 

Date of data collection: 07/10/2021

Information about geographic location of data collection: University of Southampton, Southampton, SO17 1BJ, UK
 
Licence: CC-BY

Related projects:
EPSRC grant EP/N03368X/1
EPSRC grant EP/T026197/1


Date that the file was created: 06, 2022