The University of Southampton
University of Southampton Institutional Repository

Convective tissue movements play a major role in avian endocardial morphogenesis.

Convective tissue movements play a major role in avian endocardial morphogenesis.
Convective tissue movements play a major role in avian endocardial morphogenesis.
Endocardial cells play a critical role in cardiac development and function, forming the innermost layer of the early (tubular) heart, separated from the myocardium by extracellular matrix (ECM). However, knowledge is limited regarding the interactions of cardiac progenitors and surrounding ECM during dramatic tissue rearrangements and concomitant cellular repositioning events that underlie endocardial morphogenesis. By analyzing the movements of immunolabeled ECM components (fibronectin, fibrillin-2) and TIE1 positive endocardial progenitors in time-lapse recordings of quail embryonic development, we demonstrate that the transformation of the primary heart field within the anterior lateral plate mesoderm (LPM) into a tubular heart involves the precise co-movement of primordial endocardial cells with the surrounding ECM. Thus, the ECM of the tubular heart contains filaments that were associated with the anterior LPM at earlier developmental stages. Moreover, endocardial cells exhibit surprisingly little directed active motility, that is, sustained directed movements relative to the surrounding ECM microenvironment. These findings point to the importance of large-scale tissue movements that convect cells to the appropriate positions during cardiac organogenesis.

Highlights
Endocardium, Embryo, morphogenesis, Quail, time-lapse, cardiovascular, imagining
0012-1606
348-361
Aleksandrova, Anastasiia
1c430adf-7962-4709-99a9-d71a78c35d9a
Czirók, Andras
95026253-dd04-4855-88b6-cdf4dda2465e
Szabó, Andras
fe3abc47-3aa2-441a-a9f8-5224cf4b7659
Filla, MB
69e4de3f-d42a-47a6-b388-621dc921ddae
Hossain, MJ
bba1b875-7604-462b-a55b-ba0b54f728e8
Whelan, Paul F.
80bc79fe-b56c-4c16-a794-998e8fe580cc
Lansford, Rusty
6dafd067-9ffe-47b0-914d-65c14e4b01f1
Rongish, BJ
840f8243-5710-4402-a5e2-27c4cc95171a
Aleksandrova, Anastasiia
1c430adf-7962-4709-99a9-d71a78c35d9a
Czirók, Andras
95026253-dd04-4855-88b6-cdf4dda2465e
Szabó, Andras
fe3abc47-3aa2-441a-a9f8-5224cf4b7659
Filla, MB
69e4de3f-d42a-47a6-b388-621dc921ddae
Hossain, MJ
bba1b875-7604-462b-a55b-ba0b54f728e8
Whelan, Paul F.
80bc79fe-b56c-4c16-a794-998e8fe580cc
Lansford, Rusty
6dafd067-9ffe-47b0-914d-65c14e4b01f1
Rongish, BJ
840f8243-5710-4402-a5e2-27c4cc95171a

Aleksandrova, Anastasiia, Czirók, Andras, Szabó, Andras, Filla, MB, Hossain, MJ, Whelan, Paul F., Lansford, Rusty and Rongish, BJ (2012) Convective tissue movements play a major role in avian endocardial morphogenesis. Developmental Biology, 363 (2), 348-361. (doi:10.1016/j.ydbio.2011.12.036).

Record type: Article

Abstract

Endocardial cells play a critical role in cardiac development and function, forming the innermost layer of the early (tubular) heart, separated from the myocardium by extracellular matrix (ECM). However, knowledge is limited regarding the interactions of cardiac progenitors and surrounding ECM during dramatic tissue rearrangements and concomitant cellular repositioning events that underlie endocardial morphogenesis. By analyzing the movements of immunolabeled ECM components (fibronectin, fibrillin-2) and TIE1 positive endocardial progenitors in time-lapse recordings of quail embryonic development, we demonstrate that the transformation of the primary heart field within the anterior lateral plate mesoderm (LPM) into a tubular heart involves the precise co-movement of primordial endocardial cells with the surrounding ECM. Thus, the ECM of the tubular heart contains filaments that were associated with the anterior LPM at earlier developmental stages. Moreover, endocardial cells exhibit surprisingly little directed active motility, that is, sustained directed movements relative to the surrounding ECM microenvironment. These findings point to the importance of large-scale tissue movements that convect cells to the appropriate positions during cardiac organogenesis.

Highlights

This record has no associated files available for download.

More information

Accepted/In Press date: 14 December 2011
e-pub ahead of print date: 4 January 2012
Published date: 15 March 2012
Additional Information: Copyright © 2012 Elsevier Inc.
Keywords: Endocardium, Embryo, morphogenesis, Quail, time-lapse, cardiovascular, imagining

Identifiers

Local EPrints ID: 458218
URI: http://eprints.soton.ac.uk/id/eprint/458218
ISSN: 0012-1606
PURE UUID: 18ddc848-c9f2-4431-9f36-7be08aaf2a1a
ORCID for MJ Hossain: ORCID iD orcid.org/0000-0003-3303-5755

Catalogue record

Date deposited: 01 Jul 2022 16:32
Last modified: 02 Jul 2022 02:12

Export record

Altmetrics

Contributors

Author: Anastasiia Aleksandrova
Author: Andras Czirók
Author: Andras Szabó
Author: MB Filla
Author: MJ Hossain ORCID iD
Author: Paul F. Whelan
Author: Rusty Lansford
Author: BJ Rongish

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×