A super-resolution platform for correlative live single-molecule imaging and STED microscopy
A super-resolution platform for correlative live single-molecule imaging and STED microscopy
Super-resolution microscopy offers tremendous opportunities to unravel the complex and dynamic architecture of living cells. However, current super-resolution microscopes are well suited for revealing protein distributions or cell morphology, but not both. We present a super-resolution platform that permits correlative single-molecule imaging and stimulated emission depletion microscopy in live cells. It gives nanoscale access to the positions and movements of synaptic proteins within the morphological context of growth cones and dendritic spines.
1263-1268
Inavalli, V. V. G. Krishna
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Lenz, Martin O.
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Butler, Corey
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Angibaud, Julie
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Compans, Benjamin
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Levet, Florian
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Tønnesen, Jan
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Rossier, Olivier
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Giannone, Gregory
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Thoumine, Olivier
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Hosy, Eric
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Choquet, Daniel
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Sibarita, Jean-baptiste
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Nägerl, U. Valentin
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1 December 2019
Inavalli, V. V. G. Krishna
db7ab576-a272-4f04-b938-3d1772ffbd01
Lenz, Martin O.
3978d5a7-7d7b-4d8e-ae80-24da8b9ae58f
Butler, Corey
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Angibaud, Julie
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Compans, Benjamin
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Levet, Florian
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Tønnesen, Jan
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Rossier, Olivier
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Giannone, Gregory
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Thoumine, Olivier
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Hosy, Eric
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Choquet, Daniel
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Sibarita, Jean-baptiste
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Nägerl, U. Valentin
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Inavalli, V. V. G. Krishna, Lenz, Martin O., Butler, Corey, Angibaud, Julie, Compans, Benjamin, Levet, Florian, Tønnesen, Jan, Rossier, Olivier, Giannone, Gregory, Thoumine, Olivier, Hosy, Eric, Choquet, Daniel, Sibarita, Jean-baptiste and Nägerl, U. Valentin
(2019)
A super-resolution platform for correlative live single-molecule imaging and STED microscopy.
Nature Methods, 16 (12), .
(doi:10.1038/s41592-019-0611-8).
Abstract
Super-resolution microscopy offers tremendous opportunities to unravel the complex and dynamic architecture of living cells. However, current super-resolution microscopes are well suited for revealing protein distributions or cell morphology, but not both. We present a super-resolution platform that permits correlative single-molecule imaging and stimulated emission depletion microscopy in live cells. It gives nanoscale access to the positions and movements of synaptic proteins within the morphological context of growth cones and dendritic spines.
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Accepted/In Press date: 13 September 2019
e-pub ahead of print date: 21 October 2019
Published date: 1 December 2019
Identifiers
Local EPrints ID: 458250
URI: http://eprints.soton.ac.uk/id/eprint/458250
ISSN: 1548-7091
PURE UUID: 12604d76-5888-49e2-8352-f99924b1931f
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Date deposited: 04 Jul 2022 16:44
Last modified: 17 Mar 2024 04:04
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Contributors
Author:
V. V. G. Krishna Inavalli
Author:
Martin O. Lenz
Author:
Corey Butler
Author:
Julie Angibaud
Author:
Benjamin Compans
Author:
Florian Levet
Author:
Jan Tønnesen
Author:
Olivier Rossier
Author:
Gregory Giannone
Author:
Olivier Thoumine
Author:
Eric Hosy
Author:
Daniel Choquet
Author:
Jean-baptiste Sibarita
Author:
U. Valentin Nägerl
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