A study of human epidermal growth factor and related substances in carcinomas
A study of human epidermal growth factor and related substances in carcinomas
The recognition of transforming growth factor α (TGFα) in malignancy, and its structural relationship to epidermal growth factor (EGF) has resulted in autocrine and paracrine hypotheses for growth stimulation in cancer. Previous studies have suggested that urinary levels of both these factors may be elevated in malignancy and provide a useful tumour marker. This study was undertaken to study their presence in various carcinomas. Urinary human EGF (hEGF) levels were measured by a specific 2-site ELISA. No clinically useful differences were observed between normal subjects and patients with colorectal or breast carcinomas. The presence of mitogenic activity other than hEGF in the urine was examined by immuno-absorbing hEGF using a monoclonal antibody which removes over 99% of hEGF. Immuno-absorbed urines were assayed for other growth factors by competitive binding to EGF receptors and by induction of DNA synthesis in density-inhibited human foreskin fibroblasts. No such activity was found, either in the whole curves, or after fractionation or anion exchange chromatography (FPLC). Monoclonal antibodies to hEGF and TGFα were used for immunoperoxidase staining of specimens of colorectal, bladder and renal carcinomas. Positive staining for both factors was particularly common in colorectal carcinomas, where it was localised both to the malignant epithelium and the tumour macrophages. Colorectal carcinomas were extracted using acid and detergent solutions, and the extracts assayed for mitogenic activity. Such activity was identified by the use of various techniques as EGF- and TGFα-like, TGFβ-like and fibroblast growth factor-like. Localisation of growth factors within tumour epithelium and macrophages indicates possible autocrine and paracrine mechanisms of growth stimulation.
University of Southampton
Sweetenham, J. W
4ea41ea4-f6aa-43c2-bb4c-804832d980e5
1989
Sweetenham, J. W
4ea41ea4-f6aa-43c2-bb4c-804832d980e5
Sweetenham, J. W
(1989)
A study of human epidermal growth factor and related substances in carcinomas.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The recognition of transforming growth factor α (TGFα) in malignancy, and its structural relationship to epidermal growth factor (EGF) has resulted in autocrine and paracrine hypotheses for growth stimulation in cancer. Previous studies have suggested that urinary levels of both these factors may be elevated in malignancy and provide a useful tumour marker. This study was undertaken to study their presence in various carcinomas. Urinary human EGF (hEGF) levels were measured by a specific 2-site ELISA. No clinically useful differences were observed between normal subjects and patients with colorectal or breast carcinomas. The presence of mitogenic activity other than hEGF in the urine was examined by immuno-absorbing hEGF using a monoclonal antibody which removes over 99% of hEGF. Immuno-absorbed urines were assayed for other growth factors by competitive binding to EGF receptors and by induction of DNA synthesis in density-inhibited human foreskin fibroblasts. No such activity was found, either in the whole curves, or after fractionation or anion exchange chromatography (FPLC). Monoclonal antibodies to hEGF and TGFα were used for immunoperoxidase staining of specimens of colorectal, bladder and renal carcinomas. Positive staining for both factors was particularly common in colorectal carcinomas, where it was localised both to the malignant epithelium and the tumour macrophages. Colorectal carcinomas were extracted using acid and detergent solutions, and the extracts assayed for mitogenic activity. Such activity was identified by the use of various techniques as EGF- and TGFα-like, TGFβ-like and fibroblast growth factor-like. Localisation of growth factors within tumour epithelium and macrophages indicates possible autocrine and paracrine mechanisms of growth stimulation.
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Published date: 1989
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Local EPrints ID: 458284
URI: http://eprints.soton.ac.uk/id/eprint/458284
PURE UUID: 3b547f85-80c7-449e-977d-33c14370ee92
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Date deposited: 04 Jul 2022 16:46
Last modified: 23 Jul 2022 00:15
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Author:
J. W Sweetenham
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