Expression of Alpha-1-Antitrypsin in T-cell lymphocytes
Expression of Alpha-1-Antitrypsin in T-cell lymphocytes
I have studied immunoblastic T-cell lymphoma and enteropathy associated T-cell lymphoma using immunohistochemical techniques for the demonstration of AlAT. Cytoplasmic staining for AlAT is present in the malignant cells of both types of T-cell neoplasm which also express CD30 and CD25. Peripheral blood T-lymphocytes on stimulation with mitogen also show granular cytoplasmic expression of AlAT. Time course studies show that this parallels the expression of CD30 and CD25, markers of lymphoid activation. AlAT expression appears therefore to be associated with activation in T-cells. Further studies of subfractionated T-lymphocytes suggest that the expression of AlAT on activation is not restricted to an individual lymphocyte subset. This study demonstrates AlAT mRNA in monocytes, granulocytes and lumphocytes stimulated with con-A, using synthetic oligonucleotide gene probes. Our results confirm AlAT synthesis by these cells. These cells also express exons A and B, exons not expressed in the hepatocyte. Using probes for the individual exons we have demonstrated alternative splicing of exon B in monocytes and lymphocytes.
University of Southampton
1990
Bashir, Muhammad Shahid
(1990)
Expression of Alpha-1-Antitrypsin in T-cell lymphocytes.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
I have studied immunoblastic T-cell lymphoma and enteropathy associated T-cell lymphoma using immunohistochemical techniques for the demonstration of AlAT. Cytoplasmic staining for AlAT is present in the malignant cells of both types of T-cell neoplasm which also express CD30 and CD25. Peripheral blood T-lymphocytes on stimulation with mitogen also show granular cytoplasmic expression of AlAT. Time course studies show that this parallels the expression of CD30 and CD25, markers of lymphoid activation. AlAT expression appears therefore to be associated with activation in T-cells. Further studies of subfractionated T-lymphocytes suggest that the expression of AlAT on activation is not restricted to an individual lymphocyte subset. This study demonstrates AlAT mRNA in monocytes, granulocytes and lumphocytes stimulated with con-A, using synthetic oligonucleotide gene probes. Our results confirm AlAT synthesis by these cells. These cells also express exons A and B, exons not expressed in the hepatocyte. Using probes for the individual exons we have demonstrated alternative splicing of exon B in monocytes and lymphocytes.
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Published date: 1990
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Local EPrints ID: 458311
URI: http://eprints.soton.ac.uk/id/eprint/458311
PURE UUID: f0d8921b-2573-40f8-b79c-1771254db3d9
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Date deposited: 04 Jul 2022 16:46
Last modified: 04 Jul 2022 16:46
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Author:
Muhammad Shahid Bashir
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