An experimental investigation of the cutaneous late phase response in humans
An experimental investigation of the cutaneous late phase response in humans
An inflammatory infiltrate was observed close to the dermal capillaries at the allergen-, histamine- and saline-challenged sites. The infiltrate consisted of a number of CD3+ cells. CD4+ cells showed a similar pattern and were observed in greater numbers than CD8+ cells. The majority of the CD4+ cells were antigen experienced (CD45RO+ ), suggesting an activated T cell population. A number of resident mast cells and macrophages were observed but neutrophils and significant numbers of activated eosinophils were only present in the allergen challenged biopsies, implying the accumulation of these cell types is an antigen-dependent process, unlike that of T cells. Antihistamine inhibited the early response in the skin but did not significantly effect the late response. Corticosteroid significantly inhibited the recruitment of eosinophils in the allergen challenged biopsies and tended to inhibit neutrophil influx in these biopsies. IL-1α/β bioactive peptide and IL-1β mRNA was observed in the inflammatory infiltrate localised to macrophages and IL-4 bioactive peptide was observed in association with mast cells within the inflammatory infiltrate. Few mast cells were positive for IL-4 mRNA which appeared to localise elsewhere, possibly in T cells.
These findings suggest that the recruitment of T lymphocyte to the site of the late phase response in the skin is an antigen-independent event, but that the recruitment of eosinophils and possibly neutrophils is an antigen-dependent process. The localisation of cytokine bioactive peptide and mRNA to mast cells and macrophages suggests a more prominent role for these accessory cells in the development of the late phase skin reaction. This contrasts to the view that the T lymphocytes are the primary source of pro-inflammatory cytokines in the late response and could have implications in the targeting of therapies for the treatment of a variety of chronic allergic diseases.
University of Southampton
1993
Smith, Lance Malcolm
(1993)
An experimental investigation of the cutaneous late phase response in humans.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
An inflammatory infiltrate was observed close to the dermal capillaries at the allergen-, histamine- and saline-challenged sites. The infiltrate consisted of a number of CD3+ cells. CD4+ cells showed a similar pattern and were observed in greater numbers than CD8+ cells. The majority of the CD4+ cells were antigen experienced (CD45RO+ ), suggesting an activated T cell population. A number of resident mast cells and macrophages were observed but neutrophils and significant numbers of activated eosinophils were only present in the allergen challenged biopsies, implying the accumulation of these cell types is an antigen-dependent process, unlike that of T cells. Antihistamine inhibited the early response in the skin but did not significantly effect the late response. Corticosteroid significantly inhibited the recruitment of eosinophils in the allergen challenged biopsies and tended to inhibit neutrophil influx in these biopsies. IL-1α/β bioactive peptide and IL-1β mRNA was observed in the inflammatory infiltrate localised to macrophages and IL-4 bioactive peptide was observed in association with mast cells within the inflammatory infiltrate. Few mast cells were positive for IL-4 mRNA which appeared to localise elsewhere, possibly in T cells.
These findings suggest that the recruitment of T lymphocyte to the site of the late phase response in the skin is an antigen-independent event, but that the recruitment of eosinophils and possibly neutrophils is an antigen-dependent process. The localisation of cytokine bioactive peptide and mRNA to mast cells and macrophages suggests a more prominent role for these accessory cells in the development of the late phase skin reaction. This contrasts to the view that the T lymphocytes are the primary source of pro-inflammatory cytokines in the late response and could have implications in the targeting of therapies for the treatment of a variety of chronic allergic diseases.
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Published date: 1993
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Local EPrints ID: 458381
URI: http://eprints.soton.ac.uk/id/eprint/458381
PURE UUID: a5c0dfe5-4df4-477b-86e0-f319658a334a
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Date deposited: 04 Jul 2022 16:48
Last modified: 04 Jul 2022 16:48
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Author:
Lance Malcolm Smith
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