Effects of ageing on primary and secondary transport processes in red beet (Beta vulgaris) storage tissue
Effects of ageing on primary and secondary transport processes in red beet (Beta vulgaris) storage tissue
Tonoplast- and plasma membrane- enriched fractions were prepared from fresh and aged red beet storage tissue by discontinuous sucrose centrifugation and aqueous two phase partitioning, respectively. Activity of the P-type H+ATPase, after an initial lag phase of 1 d, showed a dramatic increase in activity during 3 d of ageing. Increased activity of the plasma membrane (PM) ATPase in response to ageing was further reflected in enhanced proton pumping activity in microsomal fractions isolated from aged tissue(2 d), and Western blots of PM fractions from aged tissue (2 d) against an antibody to the PM H+ATPase. The V-ATPase and the PPase showed no significant increase in activity in response to ageing. The activity of both fluctuated during a 3d ageing period.
Uptake of 14C labelled sucrose, glucose and fructose was monitored in fresh and aged red beet discs. All three sugars showed a significant rise in uptake in response to ageing. Cordycepin and cycloheximide caused greater than 70% inhibition of induced sugar uptake into beet discs. Tunicamycin resulted in greater than 80% inhibition of inducible sugar uptake. Monensin inhibited glucose and fructose uptake into aged discs by 77% and 38% respectively, but it had no significant effect on sucrose uptake. N-Ethymaleimide inhibited both sucrose and glucose uptake. Its effect was more pronounced in fresh tissue, suggesting ageing induced protein modification. Other protein modifying reagents (diethylpyrocarbonate (DEPC), p-chloromercuribenzene sulphonic acid (PCMBS) and phenylglyoxal) although causing significant inhibition of either sucrose uptake, glucose uptake or both, showed no difference in their level of inhibition between fresh and aged tissue.
University of Southampton
Marvier, Allison Christina
1994
Marvier, Allison Christina
Marvier, Allison Christina
(1994)
Effects of ageing on primary and secondary transport processes in red beet (Beta vulgaris) storage tissue.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Tonoplast- and plasma membrane- enriched fractions were prepared from fresh and aged red beet storage tissue by discontinuous sucrose centrifugation and aqueous two phase partitioning, respectively. Activity of the P-type H+ATPase, after an initial lag phase of 1 d, showed a dramatic increase in activity during 3 d of ageing. Increased activity of the plasma membrane (PM) ATPase in response to ageing was further reflected in enhanced proton pumping activity in microsomal fractions isolated from aged tissue(2 d), and Western blots of PM fractions from aged tissue (2 d) against an antibody to the PM H+ATPase. The V-ATPase and the PPase showed no significant increase in activity in response to ageing. The activity of both fluctuated during a 3d ageing period.
Uptake of 14C labelled sucrose, glucose and fructose was monitored in fresh and aged red beet discs. All three sugars showed a significant rise in uptake in response to ageing. Cordycepin and cycloheximide caused greater than 70% inhibition of induced sugar uptake into beet discs. Tunicamycin resulted in greater than 80% inhibition of inducible sugar uptake. Monensin inhibited glucose and fructose uptake into aged discs by 77% and 38% respectively, but it had no significant effect on sucrose uptake. N-Ethymaleimide inhibited both sucrose and glucose uptake. Its effect was more pronounced in fresh tissue, suggesting ageing induced protein modification. Other protein modifying reagents (diethylpyrocarbonate (DEPC), p-chloromercuribenzene sulphonic acid (PCMBS) and phenylglyoxal) although causing significant inhibition of either sucrose uptake, glucose uptake or both, showed no difference in their level of inhibition between fresh and aged tissue.
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Published date: 1994
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Local EPrints ID: 458530
URI: http://eprints.soton.ac.uk/id/eprint/458530
PURE UUID: fb9f288a-0a9e-48b1-b951-eedfec5b7983
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Date deposited: 04 Jul 2022 16:50
Last modified: 04 Jul 2022 16:50
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Author:
Allison Christina Marvier
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