Studies on the regeneration of lens from cornea in larval Xenopus laevis
Studies on the regeneration of lens from cornea in larval Xenopus laevis
The role of the lens, inner cornea and eye cup in the control of of in vivo lens regeneration from the cornea of larval Xenopuc laevis has been investigated.Experiments involving wounding of both outer and inner corneas, lens dislocation and lensectomy followed by re-implantation of the lend showed that lone, regeneration from the cornea could 'occur in the presence of the existing lens.Results from eye cup removal and corneal transplantation experiments suggest that a stimulatory factor emanating from the eye cup: is responsible for the initiation and maintenance of corneal lens regeneration. The retina has been shown to he the source of this factor. Experimental and ultrastructural evidence is presented indicating that the Inner cornea is a barrier to the passage of retinal factor to the outer cornea thur preventing the initiation or lens regeneration and maintaining the single lens structure of the eye. Larval pituitary, limb blastema and limb bud have ail been shown to promote corneal transformation to lens when implanted between the inner and outer corneas of the eye. Immunodiffusion studies revealed some antigenic identity between extracts of pituitary, limb bud and retina.Optic vesicles transplanted beneath the tail ectoderm of tail bud stage embryos have been found to confer on tail ectoderm the ability to differentiate lens and to regenerate lens as well. It is suggested that events involved in lens induction from head ectoderm are similar to those involved in lens regeneration from the cornea.
University of Southampton
1978
Reeve, Julie Grace
(1978)
Studies on the regeneration of lens from cornea in larval Xenopus laevis.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The role of the lens, inner cornea and eye cup in the control of of in vivo lens regeneration from the cornea of larval Xenopuc laevis has been investigated.Experiments involving wounding of both outer and inner corneas, lens dislocation and lensectomy followed by re-implantation of the lend showed that lone, regeneration from the cornea could 'occur in the presence of the existing lens.Results from eye cup removal and corneal transplantation experiments suggest that a stimulatory factor emanating from the eye cup: is responsible for the initiation and maintenance of corneal lens regeneration. The retina has been shown to he the source of this factor. Experimental and ultrastructural evidence is presented indicating that the Inner cornea is a barrier to the passage of retinal factor to the outer cornea thur preventing the initiation or lens regeneration and maintaining the single lens structure of the eye. Larval pituitary, limb blastema and limb bud have ail been shown to promote corneal transformation to lens when implanted between the inner and outer corneas of the eye. Immunodiffusion studies revealed some antigenic identity between extracts of pituitary, limb bud and retina.Optic vesicles transplanted beneath the tail ectoderm of tail bud stage embryos have been found to confer on tail ectoderm the ability to differentiate lens and to regenerate lens as well. It is suggested that events involved in lens induction from head ectoderm are similar to those involved in lens regeneration from the cornea.
This record has no associated files available for download.
More information
Published date: 1978
Identifiers
Local EPrints ID: 458536
URI: http://eprints.soton.ac.uk/id/eprint/458536
PURE UUID: c1b40471-f5c9-450d-88a0-b8f769e5f288
Catalogue record
Date deposited: 04 Jul 2022 16:51
Last modified: 04 Jul 2022 16:51
Export record
Contributors
Author:
Julie Grace Reeve
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics