Calcium-independent desmosome adhesion : acquisition, maintenance and modulation
Calcium-independent desmosome adhesion : acquisition, maintenance and modulation
Desmosomal adhesion is known to be calcium dependent and desmosomal glycoproteins belong to the cadherin superfamily of adhesion molecules. Thus, MDCK cells in short term cultures lose cell-cell adhesion when extracellular calcium concentrations are lowered below 0.1mM, [ie adhesion is calcium dependent (Ca-Dep)], but cultures continued at confluence can achieve calcium independent (Ca-Ind) adhesion with time. This study shows that the acquisition of Ca-Ind adhesion is specific to the desmosome, occurs zonally within a confluent monolayer, and coincides with cell density and proliferation rat changes associated with monolayer maturation.
Ca-Ind adhesion can be induced prematurely by treating cells with protein kinase C (PKC) inhibitors, but is abolished when PKC is activated. Modulation of cyclic AMP-dependent enzyme (PKA) activity had no effect on Ca-Ind adhesion. Ca-Ind desmosome adhesion was also reversed when MDCK monolayers were wounded to produce cell islands. This switch to the Ca-Dep state, first occurring at the free edges and spreading as a wave to the island centre, was also blocked by PKC inhibition. These results provide the first evidence that desmosome adhesion can be regulated by a PKC-mediated signalling mechanism. Such modulations may be important in embryogenesis, pathogenesis and tumorigenesis.
University of Southampton
1994
Lloyd, Susan
(1994)
Calcium-independent desmosome adhesion : acquisition, maintenance and modulation.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Desmosomal adhesion is known to be calcium dependent and desmosomal glycoproteins belong to the cadherin superfamily of adhesion molecules. Thus, MDCK cells in short term cultures lose cell-cell adhesion when extracellular calcium concentrations are lowered below 0.1mM, [ie adhesion is calcium dependent (Ca-Dep)], but cultures continued at confluence can achieve calcium independent (Ca-Ind) adhesion with time. This study shows that the acquisition of Ca-Ind adhesion is specific to the desmosome, occurs zonally within a confluent monolayer, and coincides with cell density and proliferation rat changes associated with monolayer maturation.
Ca-Ind adhesion can be induced prematurely by treating cells with protein kinase C (PKC) inhibitors, but is abolished when PKC is activated. Modulation of cyclic AMP-dependent enzyme (PKA) activity had no effect on Ca-Ind adhesion. Ca-Ind desmosome adhesion was also reversed when MDCK monolayers were wounded to produce cell islands. This switch to the Ca-Dep state, first occurring at the free edges and spreading as a wave to the island centre, was also blocked by PKC inhibition. These results provide the first evidence that desmosome adhesion can be regulated by a PKC-mediated signalling mechanism. Such modulations may be important in embryogenesis, pathogenesis and tumorigenesis.
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Published date: 1994
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Local EPrints ID: 458550
URI: http://eprints.soton.ac.uk/id/eprint/458550
PURE UUID: 981fb58c-b420-4912-b4af-69f22f752199
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Date deposited: 04 Jul 2022 16:51
Last modified: 04 Jul 2022 16:51
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Author:
Susan Lloyd
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