The University of Southampton
University of Southampton Institutional Repository

Focal brain damage and the enhancement of experimental allergic encephalomyelitis : its relevance to multiple sclerosis

Focal brain damage and the enhancement of experimental allergic encephalomyelitis : its relevance to multiple sclerosis
Focal brain damage and the enhancement of experimental allergic encephalomyelitis : its relevance to multiple sclerosis

In this thesis the following hypotheses are tested: Firstly, focal brain damage enhances the severity of immunological damage in the cerebral hemispheres and spinal cord, secondly, drainage of brain-derived proteins and lipids via the lymphatic drainage pathways to the deep cervical lymph nodes is involved in this enhancement, and thirdly, microglia activated in cryolesion enhanced-EAE exhibit heterogeneity in morphology, function and pattern of involution.

The hypotheses are based on the following observations: a) Focal brain damage to the rat brain results in the rapid, widespread upregulation of perivascular cells, and b) interstitial fluid and possibly inflammatory cells drain via well defined lymphatic pathways to the deep cervical lymph nodes.

The results in this thesis show that: 1) Focal brain damage causes widespread enhancement of EAE, characterized by a significant increase in both the number of perivascular lymphocytic cuffs (p<0.01), and MHC II antigen expression on microglia (p<0.05) in the brain and spinal cord. 2) Morphological heterogeneity of microglia falls into 3 categories, a) around the cryolesion, b) in areas of lymphocyte infiltration, c) associated with neurons. This suggests that both the local spread of factors from the cryolesion, and neural mechanisms play a role in both the local and remote enhancement of EAE. 3) Lymphadenectomy reduces, but does not fully abolish, the cryolesion enhancement of EAE; this emphasises the role of lymphatic drainage of the brain and cervical lymph-nodes in the immunological attack on the CNS.

University of Southampton
Phillips, Marian Jean
Phillips, Marian Jean

Phillips, Marian Jean (1994) Focal brain damage and the enhancement of experimental allergic encephalomyelitis : its relevance to multiple sclerosis. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

In this thesis the following hypotheses are tested: Firstly, focal brain damage enhances the severity of immunological damage in the cerebral hemispheres and spinal cord, secondly, drainage of brain-derived proteins and lipids via the lymphatic drainage pathways to the deep cervical lymph nodes is involved in this enhancement, and thirdly, microglia activated in cryolesion enhanced-EAE exhibit heterogeneity in morphology, function and pattern of involution.

The hypotheses are based on the following observations: a) Focal brain damage to the rat brain results in the rapid, widespread upregulation of perivascular cells, and b) interstitial fluid and possibly inflammatory cells drain via well defined lymphatic pathways to the deep cervical lymph nodes.

The results in this thesis show that: 1) Focal brain damage causes widespread enhancement of EAE, characterized by a significant increase in both the number of perivascular lymphocytic cuffs (p<0.01), and MHC II antigen expression on microglia (p<0.05) in the brain and spinal cord. 2) Morphological heterogeneity of microglia falls into 3 categories, a) around the cryolesion, b) in areas of lymphocyte infiltration, c) associated with neurons. This suggests that both the local spread of factors from the cryolesion, and neural mechanisms play a role in both the local and remote enhancement of EAE. 3) Lymphadenectomy reduces, but does not fully abolish, the cryolesion enhancement of EAE; this emphasises the role of lymphatic drainage of the brain and cervical lymph-nodes in the immunological attack on the CNS.

This record has no associated files available for download.

More information

Published date: 1994

Identifiers

Local EPrints ID: 458601
URI: http://eprints.soton.ac.uk/id/eprint/458601
PURE UUID: b0a16997-8629-4ec0-a893-6ede7cdbf5b5

Catalogue record

Date deposited: 04 Jul 2022 16:52
Last modified: 04 Jul 2022 16:52

Export record

Contributors

Author: Marian Jean Phillips

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×