Production & differentiation of erythroid cells in normal and anaemic Xenopus leavis : y Vassiliki Aleprou-Marinou.
Production & differentiation of erythroid cells in normal and anaemic Xenopus leavis : y Vassiliki Aleprou-Marinou.
The control of erythropoiesis and the regulation of tadpole haemoglobin synthesis were studied in Xenopus laevis. Anaemia induced by phenylhydrazine resulted in stimulation of erythropoiesis in the liver and, toa lesser extent, the spleen. Erythroid cells released into the circulation exhibited high biosynthetic capacities. 22% of the anaemic animals showed significant enhancement in the synthesis of tadpole haemoglobin.Normal Xenopus injected with plasrua from anaemic toads developed polycythaemia. Stimulation of erythropoiesis occurred in the liver but not in the spleen. Increased synthesis of tadpole haemoglobin was found in erythroid cells in the liver. Administration of mammalian erythropoietin was effective in stimulating erythropoiesis in these animals, thus demonstrating lack of class specificity in its action. Perphenazine treatment was also found to stimulate erythropoiesis in the liver, but not in the spleen. The circulating blood cells following this treatment exhibited normal levels of DNA and protein synthesis, but enhanced RNA synthesis. 30% of such animals showed a significant increase in tadpole haemoglobin synthesis. Serum from normal Xenopus, when injected into anaemic toads, caused a reduction in vitro in DNA and protein synthesis in erythroid cells. Experiments with erythrocyte-conditioned mediums revealed that this inhibitory substance can be recovered from mature erythrocytes. Gel filtration revealed that the inhibition was due to low molecular weight molecules. Experiments with Xenopus kidney cells and lung extract revealed that the feedback inhibition is tissue specific to erythroid cells.The results are discussed in the light of theories regarding the regulation of foetal haemoglobin synthesis, and mechanisms are proposed for the control of erythropoiesis in Xenopus.
University of Southampton
Aleporu-Marinou, Vassiliki
1977
Aleporu-Marinou, Vassiliki
Aleporu-Marinou, Vassiliki
(1977)
Production & differentiation of erythroid cells in normal and anaemic Xenopus leavis : y Vassiliki Aleprou-Marinou.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The control of erythropoiesis and the regulation of tadpole haemoglobin synthesis were studied in Xenopus laevis. Anaemia induced by phenylhydrazine resulted in stimulation of erythropoiesis in the liver and, toa lesser extent, the spleen. Erythroid cells released into the circulation exhibited high biosynthetic capacities. 22% of the anaemic animals showed significant enhancement in the synthesis of tadpole haemoglobin.Normal Xenopus injected with plasrua from anaemic toads developed polycythaemia. Stimulation of erythropoiesis occurred in the liver but not in the spleen. Increased synthesis of tadpole haemoglobin was found in erythroid cells in the liver. Administration of mammalian erythropoietin was effective in stimulating erythropoiesis in these animals, thus demonstrating lack of class specificity in its action. Perphenazine treatment was also found to stimulate erythropoiesis in the liver, but not in the spleen. The circulating blood cells following this treatment exhibited normal levels of DNA and protein synthesis, but enhanced RNA synthesis. 30% of such animals showed a significant increase in tadpole haemoglobin synthesis. Serum from normal Xenopus, when injected into anaemic toads, caused a reduction in vitro in DNA and protein synthesis in erythroid cells. Experiments with erythrocyte-conditioned mediums revealed that this inhibitory substance can be recovered from mature erythrocytes. Gel filtration revealed that the inhibition was due to low molecular weight molecules. Experiments with Xenopus kidney cells and lung extract revealed that the feedback inhibition is tissue specific to erythroid cells.The results are discussed in the light of theories regarding the regulation of foetal haemoglobin synthesis, and mechanisms are proposed for the control of erythropoiesis in Xenopus.
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Published date: 1977
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Local EPrints ID: 458619
URI: http://eprints.soton.ac.uk/id/eprint/458619
PURE UUID: 6b5b0a62-e184-4b0e-8810-110f1e0bdec9
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Date deposited: 04 Jul 2022 16:52
Last modified: 04 Jul 2022 16:52
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Vassiliki Aleporu-Marinou
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