Immunoglobulin synthesis in leukaemia, lymphoma and immunodeficiency and correlation with normal lymphocyte function
Immunoglobulin synthesis in leukaemia, lymphoma and immunodeficiency and correlation with normal lymphocyte function
The synthesis and secretion of immunoglobulin (Ig) by cultured B cells from human neoplasia, immunodeficiency and normal lymphoid tissue has been investigated. The Ig light chain (LC) and heavy chain (HC) composition of the secreted material was studied in relation to stages of normal B cell maturation. Secretion of LC in large molar excess over HC was associated consistently with neoplastic lymphocytes demonstrating characteristics of immature cells. In two cases of chronic lymphocytic leukaemia (CLL), secreting free LC, pokeweed mitogen (PWM) stimulation, in vitro, induced balanced chain synthesis, confirmed by the use of anti-idiotypic antibody for precipitation. Normal lymphoid tissue was investigated for the counterparts of these free LC secreting cells. Adult peripheral blood lymphocytes synthesised balanced proportions of Ig chains. Spleen cells, however, demonstrated excess LC production associated with sub-populations of small and intermediate sized cells, probably representing early stages of maturation. As in the CLLs, PWM stimulation induced balanced LC and HC synthesis. Sub-populations of large cells, including mature plasma cells, synthesised balanced Ig. Secretion of free LC, as the predominant Ig product, was demonstrated in some cord blood lymphocyte cultures and in all cultures of liver cells from foetuses of more than 16 weeks gestation, where Ig synthesis was measurable. Two cultures of foetal cells also synthesised free p HC. Free LC secretion occurred in some cases of hypogammaglobulinaemia and related individuals with selective serum IgA deficiency. The synthesis and secretion of free LC appears to be associated predominantly with immature lymphocytes in normal adult tissue; in ontogeny; in immunodeficiency; and in neoplastic transformation. These findings are discussed in relation to current concepts of normal B cell development.
University of Southampton
Hannam-Harris, Anne Christine
1981
Hannam-Harris, Anne Christine
Hannam-Harris, Anne Christine
(1981)
Immunoglobulin synthesis in leukaemia, lymphoma and immunodeficiency and correlation with normal lymphocyte function.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The synthesis and secretion of immunoglobulin (Ig) by cultured B cells from human neoplasia, immunodeficiency and normal lymphoid tissue has been investigated. The Ig light chain (LC) and heavy chain (HC) composition of the secreted material was studied in relation to stages of normal B cell maturation. Secretion of LC in large molar excess over HC was associated consistently with neoplastic lymphocytes demonstrating characteristics of immature cells. In two cases of chronic lymphocytic leukaemia (CLL), secreting free LC, pokeweed mitogen (PWM) stimulation, in vitro, induced balanced chain synthesis, confirmed by the use of anti-idiotypic antibody for precipitation. Normal lymphoid tissue was investigated for the counterparts of these free LC secreting cells. Adult peripheral blood lymphocytes synthesised balanced proportions of Ig chains. Spleen cells, however, demonstrated excess LC production associated with sub-populations of small and intermediate sized cells, probably representing early stages of maturation. As in the CLLs, PWM stimulation induced balanced LC and HC synthesis. Sub-populations of large cells, including mature plasma cells, synthesised balanced Ig. Secretion of free LC, as the predominant Ig product, was demonstrated in some cord blood lymphocyte cultures and in all cultures of liver cells from foetuses of more than 16 weeks gestation, where Ig synthesis was measurable. Two cultures of foetal cells also synthesised free p HC. Free LC secretion occurred in some cases of hypogammaglobulinaemia and related individuals with selective serum IgA deficiency. The synthesis and secretion of free LC appears to be associated predominantly with immature lymphocytes in normal adult tissue; in ontogeny; in immunodeficiency; and in neoplastic transformation. These findings are discussed in relation to current concepts of normal B cell development.
This record has no associated files available for download.
More information
Published date: 1981
Identifiers
Local EPrints ID: 459552
URI: http://eprints.soton.ac.uk/id/eprint/459552
PURE UUID: 354cdb9d-e155-4719-8057-19975fab2bab
Catalogue record
Date deposited: 04 Jul 2022 17:13
Last modified: 04 Jul 2022 17:13
Export record
Contributors
Author:
Anne Christine Hannam-Harris
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics