Studies on the sorting out of chick embryo cells in monolayer culture
Studies on the sorting out of chick embryo cells in monolayer culture
The in vitro morphogenetic system of cell sorting out is the self reorganisation of mixed dissociated cells into regions of like cell type. Sorting out in monolayer has been shown to occur for all binary combinations of four embryonic chick cell types, 4 day limb bud mesenchyme, 7 day liver parenchyma, 10 day pigmented retina epithelium and 15 day corneal epithelium. A novel system for the study of sorting out in monolayer, consisting of culturing binary cell combinations on 1 mm circular hydrophilic regions in an otherwise hydrophobic substratum, was devised. Generally the cell types sorted out to give a 'circle-within-a-circle' configuration with a particular cell type always sorting internally with respect to the other. The 'circle-within-a-circle' pattern is consistent with an interpretation in terms of Steinberg's differential adhesion hypothesis and the following transitive hierarchy of lateral adhesiveness could be constructed: corneal epithelium - liver parenchyma pigmented retina epithelium, limb bud mesenchyme. Observation of all binary combinations in sub-confluent culture supported the above interpretation. The cell types were all shown to be mutually adhesive, the limb bud cells being less laterally adhesive than the three epithelial cell types.Estimation of the amount of fibronectin on the different cell types in vitro by immunofluorescent staining revealed no positive correlation with relative lateral adhesiveness. Study of the ultrastructural functional types present in vitro revealed that the most laterally adhesive cell types were those which possessed desmosomes. A model in which effective cell adhesiveness is considered to be comprised of the additive contributions from a number of different adhesive mechanisms is discussed.
University of Southampton
1981
Nicol, Alastair
(1981)
Studies on the sorting out of chick embryo cells in monolayer culture.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The in vitro morphogenetic system of cell sorting out is the self reorganisation of mixed dissociated cells into regions of like cell type. Sorting out in monolayer has been shown to occur for all binary combinations of four embryonic chick cell types, 4 day limb bud mesenchyme, 7 day liver parenchyma, 10 day pigmented retina epithelium and 15 day corneal epithelium. A novel system for the study of sorting out in monolayer, consisting of culturing binary cell combinations on 1 mm circular hydrophilic regions in an otherwise hydrophobic substratum, was devised. Generally the cell types sorted out to give a 'circle-within-a-circle' configuration with a particular cell type always sorting internally with respect to the other. The 'circle-within-a-circle' pattern is consistent with an interpretation in terms of Steinberg's differential adhesion hypothesis and the following transitive hierarchy of lateral adhesiveness could be constructed: corneal epithelium - liver parenchyma pigmented retina epithelium, limb bud mesenchyme. Observation of all binary combinations in sub-confluent culture supported the above interpretation. The cell types were all shown to be mutually adhesive, the limb bud cells being less laterally adhesive than the three epithelial cell types.Estimation of the amount of fibronectin on the different cell types in vitro by immunofluorescent staining revealed no positive correlation with relative lateral adhesiveness. Study of the ultrastructural functional types present in vitro revealed that the most laterally adhesive cell types were those which possessed desmosomes. A model in which effective cell adhesiveness is considered to be comprised of the additive contributions from a number of different adhesive mechanisms is discussed.
This record has no associated files available for download.
More information
Published date: 1981
Identifiers
Local EPrints ID: 459599
URI: http://eprints.soton.ac.uk/id/eprint/459599
PURE UUID: b5dee90c-97f4-46d7-b3da-0cb3d206ec64
Catalogue record
Date deposited: 04 Jul 2022 17:14
Last modified: 04 Jul 2022 17:14
Export record
Contributors
Author:
Alastair Nicol
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics