The lability of brain cholinesterases, proteins and proteases
The lability of brain cholinesterases, proteins and proteases
The activity of ac etylcholinoutoraco was determined in thirteen rogions of the young adult rat brain;following various bohavioural troatcontn anaonthosia, electrical stimulation, and shock avoidance conditioning. Initially, activity was measured in terns of protein content and changes in activity of this enzyme wore observed in several regions of the brain following these treatments. Further experiments indicated that those treatments also produced changes in protein concentration in some regions of the brain. The technique for measuring acetylcholinectoraso activity and total proteins was therefore modified and results wore oxpronood in terms of wet weight of tissue.The activity of psoudocholinestoraco, an enzyme predominantly localised in glia cells, wan also found to change in some regions following those behavioural treatments. Some of the changes observed correlated with changes in acetylcholinecterace activity while others wore independent of changes in ace tylcholinesteraco. This could indicate a selective change in glia coil metabolism following those treatments. Protoinase activity was also determined following behavioural treatments to investigate whether changes in protein content could be explained in terms of altered protein degradation. An attempt was made to fractionate brain proteins to see ifchanges in protein concentration were general or specific.
University of Southampton
Doberska, Christine Ann
ceaaaabf-3cb2-4c47-9aa1-f23828e73582
1978
Doberska, Christine Ann
ceaaaabf-3cb2-4c47-9aa1-f23828e73582
Doberska, Christine Ann
(1978)
The lability of brain cholinesterases, proteins and proteases.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The activity of ac etylcholinoutoraco was determined in thirteen rogions of the young adult rat brain;following various bohavioural troatcontn anaonthosia, electrical stimulation, and shock avoidance conditioning. Initially, activity was measured in terns of protein content and changes in activity of this enzyme wore observed in several regions of the brain following these treatments. Further experiments indicated that those treatments also produced changes in protein concentration in some regions of the brain. The technique for measuring acetylcholinectoraso activity and total proteins was therefore modified and results wore oxpronood in terms of wet weight of tissue.The activity of psoudocholinestoraco, an enzyme predominantly localised in glia cells, wan also found to change in some regions following those behavioural treatments. Some of the changes observed correlated with changes in acetylcholinecterace activity while others wore independent of changes in ace tylcholinesteraco. This could indicate a selective change in glia coil metabolism following those treatments. Protoinase activity was also determined following behavioural treatments to investigate whether changes in protein content could be explained in terms of altered protein degradation. An attempt was made to fractionate brain proteins to see ifchanges in protein concentration were general or specific.
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Published date: 1978
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Local EPrints ID: 459762
URI: http://eprints.soton.ac.uk/id/eprint/459762
PURE UUID: 81e22666-9989-49ee-aafc-95685478c8c8
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Date deposited: 04 Jul 2022 17:18
Last modified: 16 Mar 2024 18:33
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Author:
Christine Ann Doberska
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