The University of Southampton
University of Southampton Institutional Repository

Cellular effects of the anticancer drugs adriamycin and vincristine

Cellular effects of the anticancer drugs adriamycin and vincristine
Cellular effects of the anticancer drugs adriamycin and vincristine

This study had 3 objectives. (1) to determine the nature of adriamycin cytotoxicity towards A. proteus and CHO cells and to assess the relative importance of cytoplasmic and nuclear damage (2) to examine whether cell synchronisation is induced by vincristine and if so whether it is of primary importance in the response of cells exposed to both adriamycin and vincristine, and (3) to examine the nature of cellular interaction between both cytotoxics. Adriamycin was found to have a multiplicity of subcellular targets including the cell membrane, cytoplasmic organelles and nuclear structures. The importance of any one of these targets may depend on both the conditions found within the cell and the oanposition of fluids bathing the cell. The cell membrane and or cytoplasm appears to be the most important determinant of the cytotoxicity of adriamycin towards A. proteus, whereas damage to the nucleus may be more important in CHO cells. Membranal damage induced by adriamycin in CHO cells was important however. As expected, vincristine was found to block CHO cells in metaphase thereby raising the mitotic index. It did not induce SCE and the pattern of chromosomal aberrations induced by vincristine was consistent with interference with spindle dynamics rather than direct chromosomal interaction. Cells exposed to high concentrations of vincristine were found not to reenter the cell cycle after blocking. The primary response to log phase CHO cultures exposed to combinations of adriamycin and vincristine was determined by length of exposure to adriamycin. This was true in terms of cytotoxicity, ultrastructural damage, mitotic index and chromosomal aberrations. Plateau phase cultures were less dependent on drug scheduling for their response. These findings are discussed in terms of their implications for therapy.

University of Southampton
Walling, Jacqueline Mary
Walling, Jacqueline Mary

Walling, Jacqueline Mary (1983) Cellular effects of the anticancer drugs adriamycin and vincristine. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

This study had 3 objectives. (1) to determine the nature of adriamycin cytotoxicity towards A. proteus and CHO cells and to assess the relative importance of cytoplasmic and nuclear damage (2) to examine whether cell synchronisation is induced by vincristine and if so whether it is of primary importance in the response of cells exposed to both adriamycin and vincristine, and (3) to examine the nature of cellular interaction between both cytotoxics. Adriamycin was found to have a multiplicity of subcellular targets including the cell membrane, cytoplasmic organelles and nuclear structures. The importance of any one of these targets may depend on both the conditions found within the cell and the oanposition of fluids bathing the cell. The cell membrane and or cytoplasm appears to be the most important determinant of the cytotoxicity of adriamycin towards A. proteus, whereas damage to the nucleus may be more important in CHO cells. Membranal damage induced by adriamycin in CHO cells was important however. As expected, vincristine was found to block CHO cells in metaphase thereby raising the mitotic index. It did not induce SCE and the pattern of chromosomal aberrations induced by vincristine was consistent with interference with spindle dynamics rather than direct chromosomal interaction. Cells exposed to high concentrations of vincristine were found not to reenter the cell cycle after blocking. The primary response to log phase CHO cultures exposed to combinations of adriamycin and vincristine was determined by length of exposure to adriamycin. This was true in terms of cytotoxicity, ultrastructural damage, mitotic index and chromosomal aberrations. Plateau phase cultures were less dependent on drug scheduling for their response. These findings are discussed in terms of their implications for therapy.

This record has no associated files available for download.

More information

Published date: 1983

Identifiers

Local EPrints ID: 460115
URI: http://eprints.soton.ac.uk/id/eprint/460115
PURE UUID: 2949968c-82cc-4ffd-a50f-bba474d4ed9d

Catalogue record

Date deposited: 04 Jul 2022 17:55
Last modified: 04 Jul 2022 17:55

Export record

Contributors

Author: Jacqueline Mary Walling

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×