A study of the uptake of horseradish peroxidase by sympathetic neurons

Al-Khafaji, Fadhil Abbas Habib (1981) A study of the uptake of horseradish peroxidase by sympathetic neurons. University of Southampton, Doctoral Thesis.

Abstract

Blood vessels within the guinea-pig inferior mesenteric ganglion (IMG) were permeable to intravenously administered horseradish peroxidase (HRP). The capsules of the guinea-pig IMG and superior cervical ganglion, and the mouse IMG were permeable to HRP, while that of the mouse superior cervical ganglion was impermeable.Neurons and nerve processes within the guinea-pig IMG in vivo and in vitro readily endocytosed HRP using coated vesicles. During in vitro studies some UP within the perikarya was shown to undergo oetrograde axoplasmic transport in multivesicular bodies, dense bodies and elongated cisternae. Such export of HRP did not involve passage through the Colgi complex. HRP applied to the guinea-pig IMG in vivo was transported retrogradely to neurons within the corresponding dorsal root ganglia. The retrograde transport of HRP to such neurons was more prominent in experiments when the IMG was injured, probably due to the uptake of the enzyme by damaged axons. Ligation of the guinea-pig sciatic nerve was found to be an unsuitable injury for studying the retrograde HRP labelling of the cell bodies of origin of the damaged fibres. However, application of HRP to transected sciatic nerves resulted in large numbers of labelled neurons within the corresponding dorsal root and sacral sympathetic ganglia. All size categories of sensory neurons were labelled. Three hours delay before the application of HRP reduced the number of labelled neurons and the intensity of labelling of both sensory and sympathetic neuronal perikarya. Coated vesicles were demonstrated in damaged myelinated and non-myelinated fibres within the injured nerves.

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