Analysis of vertebrate desmosomes
Analysis of vertebrate desmosomes
This thesis is concerned with desmosomes, which are structures responsible for strong intercellular adhesion and are found characteristically between most epithelial cells of vertebrates. The primary aim was to produce antisera to components of isolated bovine nasal desmosomes and to assess inmunologically the distribution of cross-reacting antigens in the tissues of other vertebrate species. Secondly, to use such antisera to determine which components of the desmosome are extracellular and involved in the adhesive mechanism of this structure.
A literature review is presented outlining the research and hypotheses put forward to explain cell adhesion and the different approaches taken to isolating and examining the properties of adhesive molecules and vertebrate intercellular junctions.
Antibodies were raised against two groups of proteins 230/205Kd and 86/82Kd and three groups of glycoproteins 150Kd, 115Kd and 100Kd eluted from polyacrylamide gels of isolated desmosomal preparations. In general, cross-reacting antigens are widely distributed throughout the vertebrates, although those cross-reacting with antisera to the proteins are more highly conserved than those cross-reacting with antisera to the glycoproteins. The staining patterns produced suggested a desmosomel location for the antigens in epithelial tissues and in the cardiac intercalated discs of higher vertebrates and fish. However, in heart tissue of other lower vertebrates, the staining appears to correspond with morphologically primitive junctions.
A novel substrate adhesion involving antigens detected by the anti-86/82 sera is described in fish.
Evidence is presented demonstrating that antigens detected by anti-100 and anti-115 are found extracellularly in several epithelial cell lines. These are present on the cell surface prior to cell contact and show modulation in their accessibility to the antisera during monolayer formation.
Preliminary experiments suggest that Fab' fractions of anti-100 will inhibit desmosome formation in vitro of Madin Derby Bovine Kidney cells.
University of Southampton
Cowin, Pamela
5cb3f82d-b7ba-4c3f-86f1-461681fbe5be
1983
Cowin, Pamela
5cb3f82d-b7ba-4c3f-86f1-461681fbe5be
Garrod, David
51e21e3a-8441-40ad-a2c0-6aeebf12ffc9
Cowin, Pamela
(1983)
Analysis of vertebrate desmosomes.
University of Southampton, Doctoral Thesis, 256pp.
Record type:
Thesis
(Doctoral)
Abstract
This thesis is concerned with desmosomes, which are structures responsible for strong intercellular adhesion and are found characteristically between most epithelial cells of vertebrates. The primary aim was to produce antisera to components of isolated bovine nasal desmosomes and to assess inmunologically the distribution of cross-reacting antigens in the tissues of other vertebrate species. Secondly, to use such antisera to determine which components of the desmosome are extracellular and involved in the adhesive mechanism of this structure.
A literature review is presented outlining the research and hypotheses put forward to explain cell adhesion and the different approaches taken to isolating and examining the properties of adhesive molecules and vertebrate intercellular junctions.
Antibodies were raised against two groups of proteins 230/205Kd and 86/82Kd and three groups of glycoproteins 150Kd, 115Kd and 100Kd eluted from polyacrylamide gels of isolated desmosomal preparations. In general, cross-reacting antigens are widely distributed throughout the vertebrates, although those cross-reacting with antisera to the proteins are more highly conserved than those cross-reacting with antisera to the glycoproteins. The staining patterns produced suggested a desmosomel location for the antigens in epithelial tissues and in the cardiac intercalated discs of higher vertebrates and fish. However, in heart tissue of other lower vertebrates, the staining appears to correspond with morphologically primitive junctions.
A novel substrate adhesion involving antigens detected by the anti-86/82 sera is described in fish.
Evidence is presented demonstrating that antigens detected by anti-100 and anti-115 are found extracellularly in several epithelial cell lines. These are present on the cell surface prior to cell contact and show modulation in their accessibility to the antisera during monolayer formation.
Preliminary experiments suggest that Fab' fractions of anti-100 will inhibit desmosome formation in vitro of Madin Derby Bovine Kidney cells.
Text
Cowin 1983 Thesis
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Published date: 1983
Identifiers
Local EPrints ID: 460331
URI: http://eprints.soton.ac.uk/id/eprint/460331
PURE UUID: 4955c33d-5f68-45f3-83bd-175062013f1d
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Date deposited: 04 Jul 2022 18:18
Last modified: 16 Mar 2024 18:37
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Contributors
Author:
Pamela Cowin
Thesis advisor:
David Garrod
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