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The cloning, expression and characterisation of Streptococcal protein G

The cloning, expression and characterisation of Streptococcal protein G
The cloning, expression and characterisation of Streptococcal protein G

The gene for a immunoglobulin-binding protein (SPG) was cloned from Streptococcus G148 on a 4.2Kb Hindlll restriction fragment. This was achieved by Southern blot analysis using two synthetic oligonucleotides complementary to regions within the gene. The DNA sequence revealed the gene to be identical to that previously published (Olsson et at., 1987

University of Southampton
Murphy, Jonathan Paul
88f8874b-1236-4045-a253-0966a0cd94ef
Murphy, Jonathan Paul
88f8874b-1236-4045-a253-0966a0cd94ef

Murphy, Jonathan Paul (1991) The cloning, expression and characterisation of Streptococcal protein G. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

The gene for a immunoglobulin-binding protein (SPG) was cloned from Streptococcus G148 on a 4.2Kb Hindlll restriction fragment. This was achieved by Southern blot analysis using two synthetic oligonucleotides complementary to regions within the gene. The DNA sequence revealed the gene to be identical to that previously published (Olsson et at., 1987

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Published date: 1991

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Local EPrints ID: 460352
URI: http://eprints.soton.ac.uk/id/eprint/460352
PURE UUID: bd46a7ce-2aa0-4e17-8ae5-e87d91adc8e9

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Date deposited: 04 Jul 2022 18:19
Last modified: 22 Feb 2023 18:54

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Contributors

Author: Jonathan Paul Murphy

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