Genetic and immunological studies on the expression in Escherichia coli of the class 1 outer membrane protein from Neisseria meningitidis
Genetic and immunological studies on the expression in Escherichia coli of the class 1 outer membrane protein from Neisseria meningitidis
Meningoccal meningitis remains a severe life-threatening infection. Present vaccines to combat the disease consist of purified capsular polysac-charides and provide only limited protection against serogroups A & C, and give no protection against group B mcningococci. The class 1 outer membrane protein, may offer an alternative as an immunogen. Unfortunately, other components in the current experimental subcapsular vaccines, and the difficulty of obtaining class 1 protein in a native form, has hampered further investigation of its potential use in vaccines. This project uses recombinant techniques to express the class 1 protein in an E.coli host as the first step in production of an improved immunogen.
The entire class 1 gene porA had been cloned and sequenced and transferred to a plasmid vector pMTL20 (Barlow et a/., (1987
University of Southampton
White, Deborah Ann
8427772f-813c-4091-b245-b289b783dc08
1991
White, Deborah Ann
8427772f-813c-4091-b245-b289b783dc08
White, Deborah Ann
(1991)
Genetic and immunological studies on the expression in Escherichia coli of the class 1 outer membrane protein from Neisseria meningitidis.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Meningoccal meningitis remains a severe life-threatening infection. Present vaccines to combat the disease consist of purified capsular polysac-charides and provide only limited protection against serogroups A & C, and give no protection against group B mcningococci. The class 1 outer membrane protein, may offer an alternative as an immunogen. Unfortunately, other components in the current experimental subcapsular vaccines, and the difficulty of obtaining class 1 protein in a native form, has hampered further investigation of its potential use in vaccines. This project uses recombinant techniques to express the class 1 protein in an E.coli host as the first step in production of an improved immunogen.
The entire class 1 gene porA had been cloned and sequenced and transferred to a plasmid vector pMTL20 (Barlow et a/., (1987
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Published date: 1991
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Local EPrints ID: 460404
URI: http://eprints.soton.ac.uk/id/eprint/460404
PURE UUID: f76aab49-b16f-480d-9f4f-4214da524572
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Date deposited: 04 Jul 2022 18:21
Last modified: 22 Feb 2023 18:54
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Author:
Deborah Ann White
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