An electrophysiological study of rat hippocampal function using the in vitro slice preparation
An electrophysiological study of rat hippocampal function using the in vitro slice preparation
A number of electrophysiological techniques have been employed to study synaptic function in the rat hippocampal slice preparation. The project had 3 components:
In the first, a method was developed for acutely isolating hippocampal pyramidal cells from the adult rat. This was designed to produce cells which would allow investigation, using patch-clamp techniques, of ionic cell currents in single neurones.
The second project was an extracellular electrophysiological study of CA1 pyramidal cell region in the slice. A paired-pulse protocol was used to investigate differences in synaptic inhibition between septa] and temporal poles of the hippocampus. The septal pole was found to have significantly greater inhibition and also exhibited a greater ability to maintain Long Term Potentiation (LTP), a proposed physiological correlate of memory (Bliss & Lomo, 1973).
The third was an investigation of the excitatory amino acid receptors responsible for epileptiform bursting activity in 2 animal models of epilepsy: a chronic model of temporal lobe epilepsy, involved prior lesioning of the hippocampus with kainic acid, and an acute model which involved application of the GABA-A receptor antagonist, bicuculline.
University of Southampton
Williamson, Robert
ee281d55-6fd4-4abd-ad31-18947ad60c98
1991
Williamson, Robert
ee281d55-6fd4-4abd-ad31-18947ad60c98
Williamson, Robert
(1991)
An electrophysiological study of rat hippocampal function using the in vitro slice preparation.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
A number of electrophysiological techniques have been employed to study synaptic function in the rat hippocampal slice preparation. The project had 3 components:
In the first, a method was developed for acutely isolating hippocampal pyramidal cells from the adult rat. This was designed to produce cells which would allow investigation, using patch-clamp techniques, of ionic cell currents in single neurones.
The second project was an extracellular electrophysiological study of CA1 pyramidal cell region in the slice. A paired-pulse protocol was used to investigate differences in synaptic inhibition between septa] and temporal poles of the hippocampus. The septal pole was found to have significantly greater inhibition and also exhibited a greater ability to maintain Long Term Potentiation (LTP), a proposed physiological correlate of memory (Bliss & Lomo, 1973).
The third was an investigation of the excitatory amino acid receptors responsible for epileptiform bursting activity in 2 animal models of epilepsy: a chronic model of temporal lobe epilepsy, involved prior lesioning of the hippocampus with kainic acid, and an acute model which involved application of the GABA-A receptor antagonist, bicuculline.
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Published date: 1991
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Local EPrints ID: 460486
URI: http://eprints.soton.ac.uk/id/eprint/460486
PURE UUID: e2144fb4-6e14-4c72-bbe6-c95b08b9d8a4
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Date deposited: 04 Jul 2022 18:23
Last modified: 04 Jul 2022 18:23
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Author:
Robert Williamson
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