Feeding studies of the muricid gastropod Ocenebra erinacea (Linnaeus, 1758) with ultrastructural and histochemical investigations of the ABO

Humphrey, Elaine Christina (1990) Feeding studies of the muricid gastropod Ocenebra erinacea (Linnaeus, 1758) with ultrastructural and histochemical investigations of the ABO. University of Southampton, Doctoral Thesis.

Abstract

The response to prey and the general feeding behaviour of Ocenebra erinacea was observed together with feeding rates under specific laboratory conditions of different light, temperature and salinity regimes. The ultrastructure of the accessory boring organ (ABO) was investigated by standard electron microscopical techniques and a development of the ODO method gaining a spacial interpretation of this structurally complex organ. In the full description of the ABO, septate junctions in the apical region of the ABO, and `beaded' microvilli, were recorded. A number of histochemical studies, mostly at the ultrastructural level, were undertaken including Ca-ATPase, Na-K-ATPase, acid phosphatase, adenylate cyclase, substance P, serotonin, a tracer study using horseradish peroxide and an etching study of the secretion of the ABO on the chitin/protein complex of squid pen, furthering information chronicled on the muricid ABO. The enzyme results indicated at least three domains in the plasmalemma of the epithelial cells: the apical membrane, the contralacunal membrane, and the intercolumnar membrane. Whilst investigating the developing ABO in the larvae, a method for inducing egg capsule production in the laboratory was formulated.

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