The behaviour of affinity dyes at the interface of immiscible electrolytes
The behaviour of affinity dyes at the interface of immiscible electrolytes
The triazine dyes Cibacron blue F3GA (CBA) and Procion blue MX-R, two well known affinity ligands of affinity chromatography, have been investigated with respect to their use in a biosensor. The first part of this thesis investigates the behaviour of CBA at the interface between two immiscible electrolyte solutions (ITIES) using interfacial tension, micelles, reverse micelles and vesicles and a.c. impedance. The electrocapillary behaviour of CBA modified with a C16 hydrocarbon tail showed that there was a strong potential dependent adsorption resulting in a potential region where a dye monolayer appears to exist. The adsorption behaviour was related to the Esin-Markov effect which had not been previously shown for ITIES. A spectroscopic characterization of CBA by visible spectroscopy was carried out in order to interpret the data obtained from the micellar systems where an attempt was made to utilize the liquid-liquid interfaces formed in these systems as `microscopic' ITIES. The final part of this section attempted to obtain reliable capacitance data by a.c. impedance measurements. This was achieved by the development of a new reference electrode of the second kind (silver/silver tetraphenylborate). This electrode was reversible to the ions of the organic phase base electrolyte. It enabled the measurement of the impedance by reducing the resistance of the organic luggin capillary. This resistance causes phase shifts in the a.c. signal resulting in an experimental artifact. The principle of capacitance measurement as a means of biosensing was therefore demonstrated. The final part of this thesis investigated the dyes attached to optical planar waveguides in order to assess their potential in an optoelectronic device. The derivatisation of the waveguide surfaces and measurement of the resulting spectra was achieved. (DX84123)
University of Southampton
1988
Wiles, Martin Charles
(1988)
The behaviour of affinity dyes at the interface of immiscible electrolytes.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The triazine dyes Cibacron blue F3GA (CBA) and Procion blue MX-R, two well known affinity ligands of affinity chromatography, have been investigated with respect to their use in a biosensor. The first part of this thesis investigates the behaviour of CBA at the interface between two immiscible electrolyte solutions (ITIES) using interfacial tension, micelles, reverse micelles and vesicles and a.c. impedance. The electrocapillary behaviour of CBA modified with a C16 hydrocarbon tail showed that there was a strong potential dependent adsorption resulting in a potential region where a dye monolayer appears to exist. The adsorption behaviour was related to the Esin-Markov effect which had not been previously shown for ITIES. A spectroscopic characterization of CBA by visible spectroscopy was carried out in order to interpret the data obtained from the micellar systems where an attempt was made to utilize the liquid-liquid interfaces formed in these systems as `microscopic' ITIES. The final part of this section attempted to obtain reliable capacitance data by a.c. impedance measurements. This was achieved by the development of a new reference electrode of the second kind (silver/silver tetraphenylborate). This electrode was reversible to the ions of the organic phase base electrolyte. It enabled the measurement of the impedance by reducing the resistance of the organic luggin capillary. This resistance causes phase shifts in the a.c. signal resulting in an experimental artifact. The principle of capacitance measurement as a means of biosensing was therefore demonstrated. The final part of this thesis investigated the dyes attached to optical planar waveguides in order to assess their potential in an optoelectronic device. The derivatisation of the waveguide surfaces and measurement of the resulting spectra was achieved. (DX84123)
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Published date: 1988
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Local EPrints ID: 460744
URI: http://eprints.soton.ac.uk/id/eprint/460744
PURE UUID: 092ed16d-c62e-49f8-a714-19ab0adebb06
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Date deposited: 04 Jul 2022 18:29
Last modified: 04 Jul 2022 18:29
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Author:
Martin Charles Wiles
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