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The growth of the chick femur in ova and its growth and repair in vitro and on the chorioallantoic membrane

The growth of the chick femur in ova and its growth and repair in vitro and on the chorioallantoic membrane
The growth of the chick femur in ova and its growth and repair in vitro and on the chorioallantoic membrane

The in ova growth of the femur of the chick Gallus gallus was investigated between 9 and 19 days of incubation. The growth and repair of the 14 day embryonic femur was studied in vitro and when cultured on the chorioallantoic membrane (CAM) of a host chick.

In ova, 9 day femurs underwent a 100 fold increase in wet weight, dry weight, collagen and calcium. The relationship of femur growth to embryo wet weight changed during development and consequently embryo wet weight was an unreliable indicator of femur growth. Similarly femur wet weight and all parameters in matched tibias were poor indicators of femur mineralisation. There was a poor but acceptable correlation between femur dry weight and femur mineralisation.

Hepes buffered medium was shown to be unsuitable for the culture of embryonic femurs. In bicarbonate buffered medium growth and osteogen-esis occurred and some osteoid formed. In varying media composition and culture conditions mineralisation and fracture repair were absent. Growth and osteogenesis but not mineralisation or repair were improved by adding 10% foetal calf serum but was still insignificant compared with in vivo growth.

14 day old embryonic femurs, cultured on the CAM for 10 days, underwent a 10 fold increase in wet weight, dry weight, collagen content and mineralisation. After two days of culture their growth rate and morphology were similar to in vivo growth. Contralateral femurs cultured in separate eggs grew at a similar rate indicating a persistence of intrinsic control factors. The size of skeletal tissue that can be cultured on the CAM is significantly greater than was previously accepted. Fracture repair of damaged femurs occurred with mineralised tissue formed in the fracture gap. The model is suitable for the study of skeletal cell interactions, osteoclastic invasion, the toxicity of implant materials and the quantification of skeletal tissue growth and repair.

University of Southampton
Monro, Philip Peter
Monro, Philip Peter

Monro, Philip Peter (1988) The growth of the chick femur in ova and its growth and repair in vitro and on the chorioallantoic membrane. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

The in ova growth of the femur of the chick Gallus gallus was investigated between 9 and 19 days of incubation. The growth and repair of the 14 day embryonic femur was studied in vitro and when cultured on the chorioallantoic membrane (CAM) of a host chick.

In ova, 9 day femurs underwent a 100 fold increase in wet weight, dry weight, collagen and calcium. The relationship of femur growth to embryo wet weight changed during development and consequently embryo wet weight was an unreliable indicator of femur growth. Similarly femur wet weight and all parameters in matched tibias were poor indicators of femur mineralisation. There was a poor but acceptable correlation between femur dry weight and femur mineralisation.

Hepes buffered medium was shown to be unsuitable for the culture of embryonic femurs. In bicarbonate buffered medium growth and osteogen-esis occurred and some osteoid formed. In varying media composition and culture conditions mineralisation and fracture repair were absent. Growth and osteogenesis but not mineralisation or repair were improved by adding 10% foetal calf serum but was still insignificant compared with in vivo growth.

14 day old embryonic femurs, cultured on the CAM for 10 days, underwent a 10 fold increase in wet weight, dry weight, collagen content and mineralisation. After two days of culture their growth rate and morphology were similar to in vivo growth. Contralateral femurs cultured in separate eggs grew at a similar rate indicating a persistence of intrinsic control factors. The size of skeletal tissue that can be cultured on the CAM is significantly greater than was previously accepted. Fracture repair of damaged femurs occurred with mineralised tissue formed in the fracture gap. The model is suitable for the study of skeletal cell interactions, osteoclastic invasion, the toxicity of implant materials and the quantification of skeletal tissue growth and repair.

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Published date: 1988

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Local EPrints ID: 460794
URI: http://eprints.soton.ac.uk/id/eprint/460794
PURE UUID: 52189f87-024c-4575-a0cb-632c8b9fa434

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Date deposited: 04 Jul 2022 18:29
Last modified: 04 Jul 2022 18:29

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Author: Philip Peter Monro

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