Immunological investigations of the rat metrial glands
Immunological investigations of the rat metrial glands
Monoclonal antibodies have been used to categorise the cell populations present in the metrial gland. Cell surface antigens (Ia, Thy 1.1 and leucocyte common (LC)) have been detected on various cells in the metrial gland. The granulated metrial gland (GMG) cells have been shown to express LC antigen indicating their origin from bone marrow. The distribution of immunoglobulins in paraffin sections of metrial gland tissue has been assessed using an immunoperoxidase technique. Immunoglobulin G (IgG) was demonstrated in GMG cells and extracellularly in the metrial gland, though its presence in GMG cells was fixation-dependent. Immunoglobulin distribution was also assessed in paraffin sections of uterus from non-pregnant rats, but no immunoglobulin-containing lymphoid cells were found. The IgG reaction in GMG cells was not attributable to non-immunological, ionic interactions between antibodies and cellular components. The IgG in metrial gland cell lysates has been studied using agar gel immunodiffusion tests. The quantities present varied with the degree of parity of the pregnant rat, and the IgG was attritbutable to 7S IgG and not to a non-IgG substance with cross-reactivity for IgG. Metrial gland cell lysates did not react with solubilised placental tissue from which IgG had been eluted, or solubilised embryonic tissue in immunodiffusion tests. Similar studies were also carried out on metrial gland tissue from deciduomata-bearing pseudopregnant rats. Cells expressing Ia, Thy 1.1 or LC antigens were present, and IgG was demonstrated in GMG cells. IgG was also present in lysates of metrial gland cells from deciduomata. In in vitro studies of the metrial gland of pregnant rats IgG was released into the culture medium during the first two days, and GMG cells, initially IgG-positive, became IgG-negative. Endocytosis of IgG (and albumen) by GMG cells was demonstrated in vitro and indicates a possible mode of origin for the IgG in GMG cells in vivo. (D72235/87)
University of Southampton
1986
Mitchell, Barry Seath
(1986)
Immunological investigations of the rat metrial glands.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Monoclonal antibodies have been used to categorise the cell populations present in the metrial gland. Cell surface antigens (Ia, Thy 1.1 and leucocyte common (LC)) have been detected on various cells in the metrial gland. The granulated metrial gland (GMG) cells have been shown to express LC antigen indicating their origin from bone marrow. The distribution of immunoglobulins in paraffin sections of metrial gland tissue has been assessed using an immunoperoxidase technique. Immunoglobulin G (IgG) was demonstrated in GMG cells and extracellularly in the metrial gland, though its presence in GMG cells was fixation-dependent. Immunoglobulin distribution was also assessed in paraffin sections of uterus from non-pregnant rats, but no immunoglobulin-containing lymphoid cells were found. The IgG reaction in GMG cells was not attributable to non-immunological, ionic interactions between antibodies and cellular components. The IgG in metrial gland cell lysates has been studied using agar gel immunodiffusion tests. The quantities present varied with the degree of parity of the pregnant rat, and the IgG was attritbutable to 7S IgG and not to a non-IgG substance with cross-reactivity for IgG. Metrial gland cell lysates did not react with solubilised placental tissue from which IgG had been eluted, or solubilised embryonic tissue in immunodiffusion tests. Similar studies were also carried out on metrial gland tissue from deciduomata-bearing pseudopregnant rats. Cells expressing Ia, Thy 1.1 or LC antigens were present, and IgG was demonstrated in GMG cells. IgG was also present in lysates of metrial gland cells from deciduomata. In in vitro studies of the metrial gland of pregnant rats IgG was released into the culture medium during the first two days, and GMG cells, initially IgG-positive, became IgG-negative. Endocytosis of IgG (and albumen) by GMG cells was demonstrated in vitro and indicates a possible mode of origin for the IgG in GMG cells in vivo. (D72235/87)
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Published date: 1986
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Local EPrints ID: 460799
URI: http://eprints.soton.ac.uk/id/eprint/460799
PURE UUID: f27ef7f1-dc3b-4c2a-8e70-5a90980e5d13
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Date deposited: 04 Jul 2022 18:29
Last modified: 04 Jul 2022 18:29
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Author:
Barry Seath Mitchell
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