The control of luteal function during gestation of the guinea-pig
The control of luteal function during gestation of the guinea-pig
Progesterone production in response to hCG (10~* -1 iu/ml) ± (1 M) LH (10* 1 /l)PGF (1 M) db'AMP (01
g p p ( ) a
(1 /iM), LH (10-* -1 ug/ml)±PGF2a (1 pM), d.b.c'AMP (0.1-10 raM) and isoprenah'ne (10~~^-10 fig/ml) was measured in 2 hour incubations
of luteal cell suspensions prepared from non- pregnant, pregnant and hysterectomized groups of guinea-pigs. Cells from non-pregnant, early pregnant (day 10, 15 and 20 of pregnancy) and hysterectomized groups of guinea-pigs responded to hCG and LG with a dose-dependent increase in progesterone production which could be partially inhibited by PGF3 at. Cell suspensions prepared from animals on day 15 and 20 of pregnancy were less responsive to hCG and LH than those prepared on day 10 of the oestrous cycle and day 10 of pregnancy. Cell suspensions from late pregnant animals (day 40-50 of pregnancy) did not respond to LH or hCG over the concentration range used. When luteal cell suspensions from non-pregnant, day 20 pregnant and day 45 pregnant guinea-pigs were incubated with d.b.c'AMP and isoprenaline, a dose-dependent increase in progesterone production was seen in all groups. The loss of responsiveness seen in the day 40-50 pregnant animals appeared to be specific for LH and hCG.
A radioligand-binding assay was developed to measure the characteristics of hCG-binding to guinea-pig luteal tissue. No difference in the maximum hCG-binding capacity of luteal tissue from animals on day 10 of the oestrous cycle, early pregnancy and after hysterectomy was found but luteal binding capacity was markedly reduced in late pregnancy. The decrease in gonadotrophin binding in corpora lutea from late pregnancy may explain the decreased responsiveness of the luteal cells to hCG and LH seen in vitro at this time.
University of Southampton
1987
Fysh, Mary Louise
(1987)
The control of luteal function during gestation of the guinea-pig.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Progesterone production in response to hCG (10~* -1 iu/ml) ± (1 M) LH (10* 1 /l)PGF (1 M) db'AMP (01
g p p ( ) a
(1 /iM), LH (10-* -1 ug/ml)±PGF2a (1 pM), d.b.c'AMP (0.1-10 raM) and isoprenah'ne (10~~^-10 fig/ml) was measured in 2 hour incubations
of luteal cell suspensions prepared from non- pregnant, pregnant and hysterectomized groups of guinea-pigs. Cells from non-pregnant, early pregnant (day 10, 15 and 20 of pregnancy) and hysterectomized groups of guinea-pigs responded to hCG and LG with a dose-dependent increase in progesterone production which could be partially inhibited by PGF3 at. Cell suspensions prepared from animals on day 15 and 20 of pregnancy were less responsive to hCG and LH than those prepared on day 10 of the oestrous cycle and day 10 of pregnancy. Cell suspensions from late pregnant animals (day 40-50 of pregnancy) did not respond to LH or hCG over the concentration range used. When luteal cell suspensions from non-pregnant, day 20 pregnant and day 45 pregnant guinea-pigs were incubated with d.b.c'AMP and isoprenaline, a dose-dependent increase in progesterone production was seen in all groups. The loss of responsiveness seen in the day 40-50 pregnant animals appeared to be specific for LH and hCG.
A radioligand-binding assay was developed to measure the characteristics of hCG-binding to guinea-pig luteal tissue. No difference in the maximum hCG-binding capacity of luteal tissue from animals on day 10 of the oestrous cycle, early pregnancy and after hysterectomy was found but luteal binding capacity was markedly reduced in late pregnancy. The decrease in gonadotrophin binding in corpora lutea from late pregnancy may explain the decreased responsiveness of the luteal cells to hCG and LH seen in vitro at this time.
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Published date: 1987
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Local EPrints ID: 460871
URI: http://eprints.soton.ac.uk/id/eprint/460871
PURE UUID: b5874d2c-dc5b-4d13-af3c-80ac2a7a93e2
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Date deposited: 04 Jul 2022 18:31
Last modified: 04 Jul 2022 18:31
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Author:
Mary Louise Fysh
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