Micro-fabricated polymerase chain reaction device with integrated fluorescence detection elements

Bahrami, M., Melvin, T., Wilkinson, J.S. and Evans, A.G.R. (2004) Micro-fabricated polymerase chain reaction device with integrated fluorescence detection elements. Proceedings of 15th MicroMechanics Europe Workshop, Leuven Belgium. 04 - 06 Sep 2004. pp. 203-206 .

Abstract

A micro-fabricated Polymerase chain reaction (PCR) device with integrated temperature cycling and fluorescence detection elements to be used for DNA amplification and real time DNA detection is described. Our proposed device has two fundamental functions, the first is temperature cycling in order to replicate DNA strands and the second function is optical analysis in order to detect the amplified DNA. The device consists of a chamber with fluidic connection channels etched into the silicon substrate. Temperature sensor and heaters on the backside of the device are used for temperature cycling for the PCR and a lid of anodically bonded Pyrex prevents sample evaporation and links the external capillaries to the internal fluidic connection channels. Fluorescently labelled DNAs, inside the chamber, are illuminated through the optical fibre located at the wall of the chamber and fluorescence emission is detected by the photodiode at the bottom of the chamber. The photodiode is covered by a thin film optical filter in order to block out excitation light. CdS is used for the first time for such a filter and the complete micro-fabrication process is described.

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Contributors

Author: J.S. Wilkinson

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