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Micro-fabricated polymerase chain reaction device with integrated fluorescence detection elements

Micro-fabricated polymerase chain reaction device with integrated fluorescence detection elements
Micro-fabricated polymerase chain reaction device with integrated fluorescence detection elements
A micro-fabricated Polymerase chain reaction (PCR) device with integrated temperature cycling and fluorescence detection elements to be used for DNA amplification and real time DNA detection is described. Our proposed device has two fundamental functions, the first is temperature cycling in order to replicate DNA strands and the second function is optical analysis in order to detect the amplified DNA. The device consists of a chamber with fluidic connection channels etched into the silicon substrate. Temperature sensor and heaters on the backside of the device are used for temperature cycling for the PCR and a lid of anodically bonded Pyrex prevents sample evaporation and links the external capillaries to the internal fluidic connection channels. Fluorescently labelled DNAs, inside the chamber, are illuminated through the optical fibre located at the wall of the chamber and fluorescence emission is detected by the photodiode at the bottom of the chamber. The photodiode is covered by a thin film optical filter in order to block out excitation light. CdS is used for the first time for such a filter and the complete micro-fabrication process is described.
203-206
Bahrami, M.
9120e4d5-cbab-40fe-ac61-290ec1cb262a
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Evans, A.G.R.
082f720d-3830-46d7-ba87-b058af733bc3
Bahrami, M.
9120e4d5-cbab-40fe-ac61-290ec1cb262a
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Evans, A.G.R.
082f720d-3830-46d7-ba87-b058af733bc3

Bahrami, M., Melvin, T., Wilkinson, J.S. and Evans, A.G.R. (2004) Micro-fabricated polymerase chain reaction device with integrated fluorescence detection elements. Proceedings of 15th MicroMechanics Europe Workshop. 05 - 07 Sep 2004. pp. 203-206 .

Record type: Conference or Workshop Item (Paper)

Abstract

A micro-fabricated Polymerase chain reaction (PCR) device with integrated temperature cycling and fluorescence detection elements to be used for DNA amplification and real time DNA detection is described. Our proposed device has two fundamental functions, the first is temperature cycling in order to replicate DNA strands and the second function is optical analysis in order to detect the amplified DNA. The device consists of a chamber with fluidic connection channels etched into the silicon substrate. Temperature sensor and heaters on the backside of the device are used for temperature cycling for the PCR and a lid of anodically bonded Pyrex prevents sample evaporation and links the external capillaries to the internal fluidic connection channels. Fluorescently labelled DNAs, inside the chamber, are illuminated through the optical fibre located at the wall of the chamber and fluorescence emission is detected by the photodiode at the bottom of the chamber. The photodiode is covered by a thin film optical filter in order to block out excitation light. CdS is used for the first time for such a filter and the complete micro-fabrication process is described.

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More information

Published date: 2004
Venue - Dates: Proceedings of 15th MicroMechanics Europe Workshop, 2004-09-05 - 2004-09-07

Identifiers

Local EPrints ID: 46108
URI: https://eprints.soton.ac.uk/id/eprint/46108
PURE UUID: ef0ee664-77ed-4161-bdc8-278c5ee35872
ORCID for J.S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 25 May 2007
Last modified: 14 Mar 2019 01:56

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