The effects of anoxia/hypoxia on the spinal cord
The effects of anoxia/hypoxia on the spinal cord
The histology and ultrastructural morphology of the spinal cord of the hamster Mesocricetus auratus was studied in; (1) in situ perfused fixed material; (2) immersed fixed tissue immediately after dissection; (3) immersed fixed tissue after 5 hours of perfusion with normoxic acsf. The electrophysiology of the spinal cord was also studied to test the viability of the spinal cord; (1) during 5 hours of perfusion in normoxic acsf; (2) spinal cord subjected to 13, 25, and 50 minutes of anoxia. In situ perfused fixed spinal cord showed good cytological preservation at both LM and EM levels. It stained well with a preference for basic dyes and little affinity for acidic dyes. There was an excellent capillary system. Immersed fixed tissue immediately after dissection showed affinity for both basic and acidic dyes with some swelling of the cytoplasmic organelles and the nuclear envelopes. There was a greater swelling of cytoplasmic organelles and nuclear envelopes in the spinal cord fixed after 5 hours in normoxic acsf. Spinal cord in normoxic acsf for 5 hours showed good reflex (DRR-VRR) and spontaneous activity indicating that many cells had normal electrical and synaptic activity. Spinal cord treated to anoxic acsf for 50 minutes showed no DRR-VRR electrical activity but on washing in normoxic acsf, electrical activity returned within 60 minutes. These results indicate that many nerve cells are more resistant to anoxia than had previously been thought, but that the electrical viability and the cytological preservation of neurones in the isolated preparations of the spinal cord might be increased by better dissection/isolation procedures and more complex acsf containing oxygen carriers and antioxidants. Throughout the text I have used the term anoxia to refer to a medium lacking oxygen. I have made reference to other people's work where they have used the term `hypoxia' meaning a medium containing too little oxygen. Normoxia is referred to acsf bubbled with 95% O2 5% CO2.
University of Southampton
1991
Dowlatshahi, Nita
(1991)
The effects of anoxia/hypoxia on the spinal cord.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The histology and ultrastructural morphology of the spinal cord of the hamster Mesocricetus auratus was studied in; (1) in situ perfused fixed material; (2) immersed fixed tissue immediately after dissection; (3) immersed fixed tissue after 5 hours of perfusion with normoxic acsf. The electrophysiology of the spinal cord was also studied to test the viability of the spinal cord; (1) during 5 hours of perfusion in normoxic acsf; (2) spinal cord subjected to 13, 25, and 50 minutes of anoxia. In situ perfused fixed spinal cord showed good cytological preservation at both LM and EM levels. It stained well with a preference for basic dyes and little affinity for acidic dyes. There was an excellent capillary system. Immersed fixed tissue immediately after dissection showed affinity for both basic and acidic dyes with some swelling of the cytoplasmic organelles and the nuclear envelopes. There was a greater swelling of cytoplasmic organelles and nuclear envelopes in the spinal cord fixed after 5 hours in normoxic acsf. Spinal cord in normoxic acsf for 5 hours showed good reflex (DRR-VRR) and spontaneous activity indicating that many cells had normal electrical and synaptic activity. Spinal cord treated to anoxic acsf for 50 minutes showed no DRR-VRR electrical activity but on washing in normoxic acsf, electrical activity returned within 60 minutes. These results indicate that many nerve cells are more resistant to anoxia than had previously been thought, but that the electrical viability and the cytological preservation of neurones in the isolated preparations of the spinal cord might be increased by better dissection/isolation procedures and more complex acsf containing oxygen carriers and antioxidants. Throughout the text I have used the term anoxia to refer to a medium lacking oxygen. I have made reference to other people's work where they have used the term `hypoxia' meaning a medium containing too little oxygen. Normoxia is referred to acsf bubbled with 95% O2 5% CO2.
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Published date: 1991
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Local EPrints ID: 461269
URI: http://eprints.soton.ac.uk/id/eprint/461269
PURE UUID: f8dfefd2-7b32-4f6c-82c9-9615f388e8fd
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Date deposited: 04 Jul 2022 18:41
Last modified: 04 Jul 2022 18:41
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Author:
Nita Dowlatshahi
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