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Cadmium, zinc and copper regulation involving metallothionein gene expression in the anuran X. laevis

Cadmium, zinc and copper regulation involving metallothionein gene expression in the anuran X. laevis
Cadmium, zinc and copper regulation involving metallothionein gene expression in the anuran X. laevis

(1) The toxicity of dissolved cadmium and zinc to X. laevis larvae and trout fry is compared. (2) The effect of pretreating X. laevis larvae and trout fry to sub-lethal doses of either cadmium or zinc on their survival when they are exposed to lethal levels of either of these metals is evaluated. (3) The uptake dynamics of parenterally administered cadmium and zinc by the liver and kidneys of X. laevis toadlets are determined. (4) Metallothionein synthesis and accumulation in the cytoplasm of cadmium and zinc treated toadlets is related to cadmium and zinc uptake events. (5) Cadmium and zinc are chelated by de novo synthesized metallothionein 48 hrs after administration of these metals to X. laevis toadlets. (6) Metallothionein chelation of zinc is transient, and persists for approximately 24 hrs after zinc uptake in the liver of X. laevis. (7) Hormonal action probably plays a part in zinc uptake and chelation by metallothionein. (8) Cadmium chelated by metallothionein in the liver is transported by the metallothionein molecule from the liver to the kidneys, where cadmium accumulates. (9) X. laevis expresses a single isoform of metallothionein. This protein is more basic than mammalian metallothioneins, and more susceptible to air oxidation. (10) Native X.laevis liver metallothionein is Cu-thionein, which does not require the presence of zinc to be stable. (11) A pilot study is performed to determine the ability of a mouse metallothionein-I cDNA to detect X. laevis gene sequences. (12) A model of the regulation of cadmium, copper and zinc by the liver and kidneys of X. laevis is proposed. (DX88344)

University of Southampton
Woodall, Christopher John
Woodall, Christopher John

Woodall, Christopher John (1988) Cadmium, zinc and copper regulation involving metallothionein gene expression in the anuran X. laevis. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

(1) The toxicity of dissolved cadmium and zinc to X. laevis larvae and trout fry is compared. (2) The effect of pretreating X. laevis larvae and trout fry to sub-lethal doses of either cadmium or zinc on their survival when they are exposed to lethal levels of either of these metals is evaluated. (3) The uptake dynamics of parenterally administered cadmium and zinc by the liver and kidneys of X. laevis toadlets are determined. (4) Metallothionein synthesis and accumulation in the cytoplasm of cadmium and zinc treated toadlets is related to cadmium and zinc uptake events. (5) Cadmium and zinc are chelated by de novo synthesized metallothionein 48 hrs after administration of these metals to X. laevis toadlets. (6) Metallothionein chelation of zinc is transient, and persists for approximately 24 hrs after zinc uptake in the liver of X. laevis. (7) Hormonal action probably plays a part in zinc uptake and chelation by metallothionein. (8) Cadmium chelated by metallothionein in the liver is transported by the metallothionein molecule from the liver to the kidneys, where cadmium accumulates. (9) X. laevis expresses a single isoform of metallothionein. This protein is more basic than mammalian metallothioneins, and more susceptible to air oxidation. (10) Native X.laevis liver metallothionein is Cu-thionein, which does not require the presence of zinc to be stable. (11) A pilot study is performed to determine the ability of a mouse metallothionein-I cDNA to detect X. laevis gene sequences. (12) A model of the regulation of cadmium, copper and zinc by the liver and kidneys of X. laevis is proposed. (DX88344)

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Published date: 1988

Identifiers

Local EPrints ID: 461353
URI: http://eprints.soton.ac.uk/id/eprint/461353
PURE UUID: 01c57225-1e22-418b-a3aa-c9fd4ec190c2

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Date deposited: 04 Jul 2022 18:43
Last modified: 04 Jul 2022 18:43

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Author: Christopher John Woodall

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