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T cell and macrophage differentation markers in the normal and inflamed human intestine

T cell and macrophage differentation markers in the normal and inflamed human intestine
T cell and macrophage differentation markers in the normal and inflamed human intestine

A panel of monoclonal antibodies was used to compare T cell and accessory cell subpopulations within the immature (fetal), mature and inflamed human intestine and the human fetal thymus. Within the human fetal intestine (12 to 22 weeks gestation) T cells were CD45RA+ /CD45RO-, consistent with a naive population. Addition of PWM to cultured explants of fetal small intestine induced mucosal T cells to express CD45RO indicating activation of this population. An absolute increase in accessory cells expressing CD11c,KiM8,CD68,CD64 and CD14 was found in the post-natal intestine when compared to the fetal intestine and in the mature colon a greater proportion of accessory cells expressed CD64 than in the jejunum. A possible early lesion of Crohn's disease was found to consist of CD64,KiM8,CD68 and CD11c positive active histiocytes. In contrast with the fetal intestine, mature mucosal T cells were predominantly CD45RO positive memory T cells. However 1 to 8% of HML1+ IELs and a small proportion of lamina propria T cells expressed CD45RA. The latter population was significantly reduced in Crohn's disease. A proportion of both fetal and adult IELs were CD7+ /CD3-, however, of the CD3+ population 1 to 18% expressed the γdeltaTCR and the remainder expressed the αbetaTCR. A significant increase in IELS bearing the γdeltaTCR was found in coeliac disease. Approximately 30% of IELs in the normal jejunum and in coeliac disease were CD11a+ , whereas CD54 was undetectable on both IELs and epithelial cells. A marked upregulation of CD54 on lamina propria endothelial and accessory cells occurred in coeliac disease. Whereas over 95% of fetal cortical thymocytes and approximately 85% of medullary thymocytes were CD45RO+ , only 29% of medullary thymocytes expressed CD45RA. Fetal thymocytes also expressed CD11a and CD54 expression was exhibited by thymic epithelial and accessory cells. The latter population could be subdivided into KiM8+ type I scavenger cortical macrophages and CD11c+ type II medullary dendritic cells.

University of Southampton
Harvey, Joanna Elizabeth
17d20f0b-0820-49ab-b097-9b005a805ffd
Harvey, Joanna Elizabeth
17d20f0b-0820-49ab-b097-9b005a805ffd

Harvey, Joanna Elizabeth (1989) T cell and macrophage differentation markers in the normal and inflamed human intestine. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

A panel of monoclonal antibodies was used to compare T cell and accessory cell subpopulations within the immature (fetal), mature and inflamed human intestine and the human fetal thymus. Within the human fetal intestine (12 to 22 weeks gestation) T cells were CD45RA+ /CD45RO-, consistent with a naive population. Addition of PWM to cultured explants of fetal small intestine induced mucosal T cells to express CD45RO indicating activation of this population. An absolute increase in accessory cells expressing CD11c,KiM8,CD68,CD64 and CD14 was found in the post-natal intestine when compared to the fetal intestine and in the mature colon a greater proportion of accessory cells expressed CD64 than in the jejunum. A possible early lesion of Crohn's disease was found to consist of CD64,KiM8,CD68 and CD11c positive active histiocytes. In contrast with the fetal intestine, mature mucosal T cells were predominantly CD45RO positive memory T cells. However 1 to 8% of HML1+ IELs and a small proportion of lamina propria T cells expressed CD45RA. The latter population was significantly reduced in Crohn's disease. A proportion of both fetal and adult IELs were CD7+ /CD3-, however, of the CD3+ population 1 to 18% expressed the γdeltaTCR and the remainder expressed the αbetaTCR. A significant increase in IELS bearing the γdeltaTCR was found in coeliac disease. Approximately 30% of IELs in the normal jejunum and in coeliac disease were CD11a+ , whereas CD54 was undetectable on both IELs and epithelial cells. A marked upregulation of CD54 on lamina propria endothelial and accessory cells occurred in coeliac disease. Whereas over 95% of fetal cortical thymocytes and approximately 85% of medullary thymocytes were CD45RO+ , only 29% of medullary thymocytes expressed CD45RA. Fetal thymocytes also expressed CD11a and CD54 expression was exhibited by thymic epithelial and accessory cells. The latter population could be subdivided into KiM8+ type I scavenger cortical macrophages and CD11c+ type II medullary dendritic cells.

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Published date: 1989

Identifiers

Local EPrints ID: 461714
URI: http://eprints.soton.ac.uk/id/eprint/461714
PURE UUID: 2cb7222e-8b5f-4f7e-8b31-e784ba4bb749

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Date deposited: 04 Jul 2022 18:52
Last modified: 23 Jul 2022 00:34

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Contributors

Author: Joanna Elizabeth Harvey

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