Hippocampal suspension grafts in the kainic acid lesioned hippocampus of the rat
Hippocampal suspension grafts in the kainic acid lesioned hippocampus of the rat
In vivo electrophysiological techniques were used to assess the long-term alterations of the rat hippocampus following an ICV injection of kainic acid (KA). A loss of paired pulse inhibition in the ipsilateral hippocampus was observed 1 week after KA injection, which showed no recovery for up to 16 weeks. In the contralateral hippocampus, paired pulse inhibition was unaltered at 1 week but was reduced at 4, 8 and 16 weeks after KA. A histological examination of KA-lesioned hippocampal sections revealed a decrease in the number of non-pyramidal cells in the stratum oriens of the CA3 and CA1 regions and in the hilar region, which was more obvious at survival times of greater than 1 week. The possibility that fetal hippocampal suspension grafts could correct the electrophysiological abnormalities of the KA-lesioned hippocampus of the rat was investigated. Fetal hippocampal cell suspensions were injected into the hippocampi of host rats 1 week after an injection of KA. After a survival period of 7-20 weeks, an in vivo paired pulse protocol was performed. In the ipsilateral hippocampus, an injection of either cells or vehicle combined with a brief electrophysiological assessment performed at 1 week produced a significant increase in the paired pulse inhibition which was lost 1 week after KA alone. A slight increase was seen after an injection of either cells or vehicle alone, while electrophysiology alone had a negligible effect. In the contralateral hippocampus, an injection of cells combined with electrophysiology prevented the decrease in paired pulse inhibition seen after KA alone, while an injection of cells alone or an injection of vehicle combined with electrophysiology had a slightly smaller effect. A variety of histological techniques were employed to demonstrate the viability of the transplanted cells and their incorporation into, and organisation within, the host hippocampus.
University of Southampton
1987
Cornish, Sheena Mary
(1987)
Hippocampal suspension grafts in the kainic acid lesioned hippocampus of the rat.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
In vivo electrophysiological techniques were used to assess the long-term alterations of the rat hippocampus following an ICV injection of kainic acid (KA). A loss of paired pulse inhibition in the ipsilateral hippocampus was observed 1 week after KA injection, which showed no recovery for up to 16 weeks. In the contralateral hippocampus, paired pulse inhibition was unaltered at 1 week but was reduced at 4, 8 and 16 weeks after KA. A histological examination of KA-lesioned hippocampal sections revealed a decrease in the number of non-pyramidal cells in the stratum oriens of the CA3 and CA1 regions and in the hilar region, which was more obvious at survival times of greater than 1 week. The possibility that fetal hippocampal suspension grafts could correct the electrophysiological abnormalities of the KA-lesioned hippocampus of the rat was investigated. Fetal hippocampal cell suspensions were injected into the hippocampi of host rats 1 week after an injection of KA. After a survival period of 7-20 weeks, an in vivo paired pulse protocol was performed. In the ipsilateral hippocampus, an injection of either cells or vehicle combined with a brief electrophysiological assessment performed at 1 week produced a significant increase in the paired pulse inhibition which was lost 1 week after KA alone. A slight increase was seen after an injection of either cells or vehicle alone, while electrophysiology alone had a negligible effect. In the contralateral hippocampus, an injection of cells combined with electrophysiology prevented the decrease in paired pulse inhibition seen after KA alone, while an injection of cells alone or an injection of vehicle combined with electrophysiology had a slightly smaller effect. A variety of histological techniques were employed to demonstrate the viability of the transplanted cells and their incorporation into, and organisation within, the host hippocampus.
This record has no associated files available for download.
More information
Published date: 1987
Identifiers
Local EPrints ID: 461892
URI: http://eprints.soton.ac.uk/id/eprint/461892
PURE UUID: 829b8476-5dea-45c6-a45b-33c3ff46d1f2
Catalogue record
Date deposited: 04 Jul 2022 18:58
Last modified: 04 Jul 2022 18:58
Export record
Contributors
Author:
Sheena Mary Cornish
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics