Erythropoiesis in Xenopus laevis during recovery from phenylhydrazine-induced anaemia
Erythropoiesis in Xenopus laevis during recovery from phenylhydrazine-induced anaemia
The present study has been concerned with the characteristics of the erythropoietic process in Xenopus laevis. Phenylhydrazine was used as a haemolytic agent; at the dosage used it caused complete destruction of circulating erythrocytes. The resultant anaemia stimulated erythropoietic activity. Examination of the blood at varying times after the induction of anaemia has shown the presence of large numbers of immature erythroid cells in the peripheral circulation; to a large extent these appear to differentiate as a cohort. The morphological-biosynthetic correlates of the cells present at different times in the recovery from anaemia have been established. Cell morphology was studied by measurements of cell dimensions in smearss and by staining characteristics. The biosynthetic capacities of the erythroid cells were determined by in vitro incubation of the cells with the radioisotopes, H3 thymidine, H3 uridine and H3 leucine, and subsequent estimation of their rates of incorporation into DNA, RNA and protein respectively, by scintillation counting and autoradiographic techniques. The origin of the immature erythroid cells which appear in the peripheral circulation was studied by preparing tissue touch imprints of potential erythropoietic organs. Evidence is presented which indicates that a population of cells presentin the livers and, to a lesser extent, the spleens of anaemic animals are the probable precursors of the circulating erythroid cells. The use of erythropoiesis as a model system for the study of the processes involved in cytodifferentiation has been considered. The advantages of using phenylhydrazine-treated Xenopus laevis as a system for the study of erythropoiesis and of differentiation, in general, has been discussed and suggestions for its further use in this respect have been made.
University of Southampton
1975
Thomas, Nesta
(1975)
Erythropoiesis in Xenopus laevis during recovery from phenylhydrazine-induced anaemia.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The present study has been concerned with the characteristics of the erythropoietic process in Xenopus laevis. Phenylhydrazine was used as a haemolytic agent; at the dosage used it caused complete destruction of circulating erythrocytes. The resultant anaemia stimulated erythropoietic activity. Examination of the blood at varying times after the induction of anaemia has shown the presence of large numbers of immature erythroid cells in the peripheral circulation; to a large extent these appear to differentiate as a cohort. The morphological-biosynthetic correlates of the cells present at different times in the recovery from anaemia have been established. Cell morphology was studied by measurements of cell dimensions in smearss and by staining characteristics. The biosynthetic capacities of the erythroid cells were determined by in vitro incubation of the cells with the radioisotopes, H3 thymidine, H3 uridine and H3 leucine, and subsequent estimation of their rates of incorporation into DNA, RNA and protein respectively, by scintillation counting and autoradiographic techniques. The origin of the immature erythroid cells which appear in the peripheral circulation was studied by preparing tissue touch imprints of potential erythropoietic organs. Evidence is presented which indicates that a population of cells presentin the livers and, to a lesser extent, the spleens of anaemic animals are the probable precursors of the circulating erythroid cells. The use of erythropoiesis as a model system for the study of the processes involved in cytodifferentiation has been considered. The advantages of using phenylhydrazine-treated Xenopus laevis as a system for the study of erythropoiesis and of differentiation, in general, has been discussed and suggestions for its further use in this respect have been made.
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Published date: 1975
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Local EPrints ID: 462323
URI: http://eprints.soton.ac.uk/id/eprint/462323
PURE UUID: 3654166b-bb96-42f5-b22b-af8618b63d4e
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Date deposited: 04 Jul 2022 19:05
Last modified: 04 Jul 2022 19:05
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Author:
Nesta Thomas
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