Introduction of novel genes into talapia (Oreochromis niloticus)
Introduction of novel genes into talapia (Oreochromis niloticus)
Five different gene constructs were successfully transferred into the tilapia genome. The genes that were used contained either mammalian promoter (mMT-1) spliced to a mammalian growth hormone gene (mMTrGH), a piscine promoter (carp β-actin) spliced to a reporter gene (CAT) (pFV-1, pFV-2) or a piscine promoter (carp β-actin or ocean pout antifreeze protein) spliced to a piscine (chinook salmon) growth hormone gene (FVcsGH, opAFPGH). Multiple copies of the foreign gene appeared to be integrated at either a single site or at multiple sites in the host genome. Transgene mosaicism was observed within one tissue as well as among different tissues of the same fish. Germline transmission of the transgene was attempted, and one fish was found to contain the transgene in its sperm, but no transmission was achieved due to extreme mosaicism. Expression of reporter genes was observed transiently as well as from putative integrated transgenes from 2 weeks old fry.
Transient expression of reporter genes injected directly into tilapia muscle was also observed. Expression was first detected 48 hours after injection. DNA analysis revealed that the transgene persisted up to 48 hours after injection. Neither the transgene DNA nor the transcription product were present 7 days after injection.
A preliminary study suggests that the transgene can be transferred through the germline by injecting the testis with the foreign DNA in vivo.
University of Southampton
1993
Rahman, Md. Azizur
(1993)
Introduction of novel genes into talapia (Oreochromis niloticus).
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Five different gene constructs were successfully transferred into the tilapia genome. The genes that were used contained either mammalian promoter (mMT-1) spliced to a mammalian growth hormone gene (mMTrGH), a piscine promoter (carp β-actin) spliced to a reporter gene (CAT) (pFV-1, pFV-2) or a piscine promoter (carp β-actin or ocean pout antifreeze protein) spliced to a piscine (chinook salmon) growth hormone gene (FVcsGH, opAFPGH). Multiple copies of the foreign gene appeared to be integrated at either a single site or at multiple sites in the host genome. Transgene mosaicism was observed within one tissue as well as among different tissues of the same fish. Germline transmission of the transgene was attempted, and one fish was found to contain the transgene in its sperm, but no transmission was achieved due to extreme mosaicism. Expression of reporter genes was observed transiently as well as from putative integrated transgenes from 2 weeks old fry.
Transient expression of reporter genes injected directly into tilapia muscle was also observed. Expression was first detected 48 hours after injection. DNA analysis revealed that the transgene persisted up to 48 hours after injection. Neither the transgene DNA nor the transcription product were present 7 days after injection.
A preliminary study suggests that the transgene can be transferred through the germline by injecting the testis with the foreign DNA in vivo.
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Published date: 1993
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Local EPrints ID: 462351
URI: http://eprints.soton.ac.uk/id/eprint/462351
PURE UUID: fbd1aea3-e476-4106-9b62-a6405c71c2da
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Date deposited: 04 Jul 2022 19:06
Last modified: 04 Jul 2022 19:06
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Author:
Md. Azizur Rahman
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