Microscale structure aludcidation of the monoterpenes in the frass of Hylotrupes Bajulus (L) (Coleoptera:Cerambyciade), and their role in oviposition attraction
Microscale structure aludcidation of the monoterpenes in the frass of Hylotrupes Bajulus (L) (Coleoptera:Cerambyciade), and their role in oviposition attraction
The work reported. in this thesis describes microscale structural elucidation studies on organic extracts of insect origin. Techniques were established' for microscale chemical analysis including gas chromatography, abstractor post-loops, syringe reactions and microozonolysis. A high sensitivity gas chromatography-mass.. spectroscopy system' was developed to operate in parallel with an existing one. An All-glass microprepaiative splitter and trapping' system was designed and used in conjunction with ultraviolet and proton Fourier transform )'U1 spectroscopy at the microgram level.'Behavioural studies on H. bajulus (L) (Coleoptera Cerambycidae)Indicated the operation of chemical factors in sexual and oviposition behaviour. The mono-oxygenated monoterpenes identified by chemical and spectroscopic analysis of frass were verbenone, 2-cymene-8-ol, myrtenol,,trans-pinocarve ol, cis-pin-3-en-2-o1, oC-terpineol, y terpinen-4-ol' and 3,6,6-trimethylcyclohepta-2,4-dienone. 'Physiological activity was followed, during purification procedures by electroantennography.IIIStatistical analysis of the bioassays with (-)-verbenone an p-cymene-8-oi implicated both:these compounds in;oviposition attraction.Solid sample gas. liqujd.chromatography showedd male and female adult beetles to have different volitile constituents.
University of Southampton
Higgs, Martin Douglas
c237d89a-12f6-42bc-af67-08c6f2e74b98
1975
Higgs, Martin Douglas
c237d89a-12f6-42bc-af67-08c6f2e74b98
Higgs, Martin Douglas
(1975)
Microscale structure aludcidation of the monoterpenes in the frass of Hylotrupes Bajulus (L) (Coleoptera:Cerambyciade), and their role in oviposition attraction.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The work reported. in this thesis describes microscale structural elucidation studies on organic extracts of insect origin. Techniques were established' for microscale chemical analysis including gas chromatography, abstractor post-loops, syringe reactions and microozonolysis. A high sensitivity gas chromatography-mass.. spectroscopy system' was developed to operate in parallel with an existing one. An All-glass microprepaiative splitter and trapping' system was designed and used in conjunction with ultraviolet and proton Fourier transform )'U1 spectroscopy at the microgram level.'Behavioural studies on H. bajulus (L) (Coleoptera Cerambycidae)Indicated the operation of chemical factors in sexual and oviposition behaviour. The mono-oxygenated monoterpenes identified by chemical and spectroscopic analysis of frass were verbenone, 2-cymene-8-ol, myrtenol,,trans-pinocarve ol, cis-pin-3-en-2-o1, oC-terpineol, y terpinen-4-ol' and 3,6,6-trimethylcyclohepta-2,4-dienone. 'Physiological activity was followed, during purification procedures by electroantennography.IIIStatistical analysis of the bioassays with (-)-verbenone an p-cymene-8-oi implicated both:these compounds in;oviposition attraction.Solid sample gas. liqujd.chromatography showedd male and female adult beetles to have different volitile constituents.
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Published date: 1975
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Local EPrints ID: 462539
URI: http://eprints.soton.ac.uk/id/eprint/462539
PURE UUID: 76f7dae3-8319-48a4-b110-d4ba4e4a42f3
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Last modified: 16 Mar 2024 18:57
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Martin Douglas Higgs
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