Isolation and antigenic characterisation of UK isolates of Borrelia burgdorferi
Isolation and antigenic characterisation of UK isolates of Borrelia burgdorferi
Lyme disease is a tick-borne bacterial infection which is caused by the spirochete Borrelia burgdorferi and transmitted by the bite of Ixodes ticks. It is a multi-system infection, and is now recognized as the most common tick-borne infection in Europe, North America, and other temperate countries. Although Lyme disease has been identified in the UK no precise epidemiological figures have been available for the UK, however the New Forest area of Hampshire has been particularly associated with the occurrence of the infection.
A serological survey was carried out to compare sera from inner city blood donors with sera from New Forest inhabitants. This demonstrated a significantly higher prevalence of antibodies reacting with B.burgdorferi in the sera from the New Forest population. Further, a comparison of the prevalence rates among residents of the New Forest with forestry workers, strongly suggested a correlation between occurrence of infection and exposure to risk of tick bites.
That B.burgdorferi is endemic in the New Forest and surrounding areas, was unequivocally confirmed by the use of the Polymerase Chain Reaction (PCR). The use of PCR with oligonucleotide primers corresponding to the B.burgdorferi OspAB operon, provided a sensitive and specific method of detecting B.burgdorferi genome in infected ticks. These studies showed widespread occurrence of infection in ticks collected throughout the New Forest and surrounding area, although large differences were seen in the infection levels in ticks collected from different locations.
The mid guts from infected Ixodes ricinus ticks were inoculated into medium to grow and successfully isolate pure cultures of two UK strains of B.burgdorferi. The availability of these UK isolates enabled their antigenic characterisation and comparison with the foreign strains currently used for serological diagnosis in the UK. These studies revealed significant antigenic differences between the strains.
University of Southampton
Sorouri-Zanjani, Rahim
20ead5fa-c2c1-4ee9-ae6f-20360aed265b
1993
Sorouri-Zanjani, Rahim
20ead5fa-c2c1-4ee9-ae6f-20360aed265b
Sorouri-Zanjani, Rahim
(1993)
Isolation and antigenic characterisation of UK isolates of Borrelia burgdorferi.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Lyme disease is a tick-borne bacterial infection which is caused by the spirochete Borrelia burgdorferi and transmitted by the bite of Ixodes ticks. It is a multi-system infection, and is now recognized as the most common tick-borne infection in Europe, North America, and other temperate countries. Although Lyme disease has been identified in the UK no precise epidemiological figures have been available for the UK, however the New Forest area of Hampshire has been particularly associated with the occurrence of the infection.
A serological survey was carried out to compare sera from inner city blood donors with sera from New Forest inhabitants. This demonstrated a significantly higher prevalence of antibodies reacting with B.burgdorferi in the sera from the New Forest population. Further, a comparison of the prevalence rates among residents of the New Forest with forestry workers, strongly suggested a correlation between occurrence of infection and exposure to risk of tick bites.
That B.burgdorferi is endemic in the New Forest and surrounding areas, was unequivocally confirmed by the use of the Polymerase Chain Reaction (PCR). The use of PCR with oligonucleotide primers corresponding to the B.burgdorferi OspAB operon, provided a sensitive and specific method of detecting B.burgdorferi genome in infected ticks. These studies showed widespread occurrence of infection in ticks collected throughout the New Forest and surrounding area, although large differences were seen in the infection levels in ticks collected from different locations.
The mid guts from infected Ixodes ricinus ticks were inoculated into medium to grow and successfully isolate pure cultures of two UK strains of B.burgdorferi. The availability of these UK isolates enabled their antigenic characterisation and comparison with the foreign strains currently used for serological diagnosis in the UK. These studies revealed significant antigenic differences between the strains.
This record has no associated files available for download.
More information
Published date: 1993
Identifiers
Local EPrints ID: 462542
URI: http://eprints.soton.ac.uk/id/eprint/462542
PURE UUID: 0ff21287-c121-46c3-b6eb-9323aec2f8a7
Catalogue record
Date deposited: 04 Jul 2022 19:18
Last modified: 22 Feb 2023 18:55
Export record
Contributors
Author:
Rahim Sorouri-Zanjani
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics